Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme

Hui Wei; Zidong Wang; Jiong Zhang; Stephen House; Yi Gui Gao; Limin Yang; Howard Robinson; Li Huey Tan; Hang Xing; Changjun Hou; Ian M. Robertson; Jian Min Zuo; Yi Lu

doi:10.1038/nnano.2010.280

Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme

Hui Wei, Zidong Wang, Jiong Zhang, Stephen House, Yi Gui Gao, Limin Yang, Howard Robinson, Li Huey Tan, Hang Xing, Changjun Hou, Ian M. Robertson, Jian Min Zuo, Yi Lu

Research output: Contribution to journal › Article › peer-review

Abstract

Gold nanoparticles are useful in biomedical applications due to their distinct optical properties and high chemical stability^1-5. Reports of the biogenic formation of gold colloids from gold complexes has also led to an increased level of interest in the biomineralization of gold^6-13. However, the mechanism responsible for biomolecule-directed gold nanoparticle formation remains unclear due to the lack of structural information about biological systems and the fast kinetics of biomimetic chemical systems in solution. Here we show that intact single crystals of lysozyme can be used to study the time-dependent, protein-directed growth of gold nanoparticles. The protein crystals slow down the growth of the gold nanoparticles, allowing detailed kinetic studies to be carried out, and permit a three-dimensional structural characterization that would be difficult to achieve in solution. Furthermore, we show that additional chemical species can be used to fine-tune the growth rate of the gold nanoparticles.

Original language	English (US)
Pages (from-to)	93-97
Number of pages	5
Journal	Nature Nanotechnology
Volume	6
Issue number	2
DOIs	https://doi.org/10.1038/nnano.2010.280
State	Published - Feb 2011

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Biomedical Engineering
General Materials Science
Condensed Matter Physics
Electrical and Electronic Engineering

Online availability

10.1038/nnano.2010.280

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@article{e4398d3687ec410ca7660aca0c1e2705,

title = "Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme",

abstract = "Gold nanoparticles are useful in biomedical applications due to their distinct optical properties and high chemical stability1-5. Reports of the biogenic formation of gold colloids from gold complexes has also led to an increased level of interest in the biomineralization of gold6-13. However, the mechanism responsible for biomolecule-directed gold nanoparticle formation remains unclear due to the lack of structural information about biological systems and the fast kinetics of biomimetic chemical systems in solution. Here we show that intact single crystals of lysozyme can be used to study the time-dependent, protein-directed growth of gold nanoparticles. The protein crystals slow down the growth of the gold nanoparticles, allowing detailed kinetic studies to be carried out, and permit a three-dimensional structural characterization that would be difficult to achieve in solution. Furthermore, we show that additional chemical species can be used to fine-tune the growth rate of the gold nanoparticles.",

author = "Hui Wei and Zidong Wang and Jiong Zhang and Stephen House and Gao, {Yi Gui} and Limin Yang and Howard Robinson and Tan, {Li Huey} and Hang Xing and Changjun Hou and Robertson, {Ian M.} and Zuo, {Jian Min} and Yi Lu",

note = "Funding Information: This work was supported by the US National Science Foundation (CMMI 0749028 and DMR-0117792). The authors thank C. Lei and J. Wen for help with the (S)TEM imaging, L.A. Miller for the preparation of microtome samples and 75 kV TEM imaging, J. Soares for solid-state absorption spectroscopic measurements, M. Sardela for XRD measurements, and Y.-W. Lin, N.M. Marshall, S.-L. Tian, H.E. Ihms and K.-D. Miner for helpful discussions. J.Z. and J.M.Z. are supported by DOE DEFG02-01ER45923. S.H. and I.M.R. acknowledge support from the US Department of Energy (grant DE-FC36-05GO15064). (S)TEM experiments were carried out in part in the Frederick Seitz Materials Research Laboratory Central Facilities, University of Illinois.",

year = "2011",

month = feb,

doi = "10.1038/nnano.2010.280",

language = "English (US)",

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AU - Wei, Hui

AU - Wang, Zidong

AU - Zhang, Jiong

AU - House, Stephen

AU - Gao, Yi Gui

AU - Yang, Limin

AU - Robinson, Howard

AU - Tan, Li Huey

AU - Xing, Hang

AU - Hou, Changjun

AU - Robertson, Ian M.

AU - Zuo, Jian Min

AU - Lu, Yi

N1 - Funding Information: This work was supported by the US National Science Foundation (CMMI 0749028 and DMR-0117792). The authors thank C. Lei and J. Wen for help with the (S)TEM imaging, L.A. Miller for the preparation of microtome samples and 75 kV TEM imaging, J. Soares for solid-state absorption spectroscopic measurements, M. Sardela for XRD measurements, and Y.-W. Lin, N.M. Marshall, S.-L. Tian, H.E. Ihms and K.-D. Miner for helpful discussions. J.Z. and J.M.Z. are supported by DOE DEFG02-01ER45923. S.H. and I.M.R. acknowledge support from the US Department of Energy (grant DE-FC36-05GO15064). (S)TEM experiments were carried out in part in the Frederick Seitz Materials Research Laboratory Central Facilities, University of Illinois.

PY - 2011/2

Y1 - 2011/2

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Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme

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