Thyroid hormone responsive (THRSP) promotes the synthesis of medium-chain fatty acids in goat mammary epithelial cells

D. W. Yao, J. Luo, Q. Y. He, M. Wu, H. B. Shi, H. Wang, M. Wang, H. F. Xu, J. J. Loor

Research output: Contribution to journalArticle

Abstract

In nonruminants, thyroid hormone responsive (THRSP) is a crucial protein for cellular de novo lipogenesis. However, the role of THRSP in regulating the synthesis of milk fatty acid composition in goat mammary gland remains unknown. In the present study, we compared gene expression of THRSP among different goat tissues. Results revealed that THRSP had the highest expression in subcutaneous fat, and expression was higher during lactation compared with the dry period. Overexpression of THRSP upregulated the expression of fatty acid synthase (FASN), stearoyl-coenzyme A desaturase 1 (SCD1), diacylglycerol acyltransferase 2 (DGAT2), and glycerol-3-phosphate acyltransferase (GPAM) in goat mammary epithelial cells. In contrast, overexpression of THRSP led to downregulation of thrombospondin receptor (CD36) and had no effect on the expression of acetyl-coenzyme A carboxylase α (ACACA) and sterol regulatory element binding transcription factor1 (SREBF1). In addition, overexpressing THRSP in vitro resulted in a significant increase in triacylglycerol (TAG) concentration and the concentrations of C12:0 and C14:0. Taken together, these results highlight an important role of THRSP in regulating lipogenesis in goat mammary epithelial cells.

Original languageEnglish (US)
Pages (from-to)3124-3133
Number of pages10
JournalJournal of Dairy Science
Volume99
Issue number4
DOIs
StatePublished - Apr 1 2016

Keywords

  • Dairy nutrition
  • Fatty acid composition
  • Goat
  • Milk fat

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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