Three-dimensionally printed biological machines powered by skeletal muscle

Caroline Cvetkovic, Ritu Raman, Vincent Chan, Brian J. Williams, Madeline Tolish, Piyush Bajaj, Mahmut Selman Sakar, H. Harry Asada, M. Taher A Saif, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

Combining biological components, such as cells and tissues, with soft robotics can enable the fabrication of biological machines with the ability to sense, process signals, and produce force. An intuitive demonstration of a biological machine is one that can produce motion in response to controllable external signaling. Whereas cardiac cell-driven biological actuators have been demonstrated, the requirements of these machines to respond to stimuli and exhibit controlled movement merit the use of skeletal muscle, the primary generator of actuation in animals, as a contractile power source. Here, we report the development of 3D printed hydrogel "bio-bots" with an asymmetric physical design and powered by the actuation of an engineered mammalian skeletal muscle strip to result in net locomotion of the bio-bot. Geometric design and material properties of the hydrogel bio-bots were optimized using stereolithographic 3D printing, and the effect of collagen I and fibrin extracellular matrix proteins and insulin-like growth factor 1 on the force production of engineered skeletal muscle was characterized. Electrical stimulation triggered contraction of cells in the muscle strip and net locomotion of the bio-bot with a maximum velocity of ∼156 μm s -1, which is over 1.5 body lengths per min. Modeling and simulation were used to understand both the effect of different design parameters on the bio-bot and the mechanism of motion. This demonstration advances the goal of realizing forward-engineered integrated cellular machines and systems, which can have a myriad array of applications in drug screening, programmable tissue engineering, drug delivery, and biomimetic machine design.

Original languageEnglish (US)
Pages (from-to)10125-10130
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number28
DOIs
StatePublished - Jul 15 2014

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Skeletal Muscle
Hydrogel
Locomotion
Electric Power Supplies
Biomimetics
Preclinical Drug Evaluations
Extracellular Matrix Proteins
Robotics
Somatomedins
Tissue Engineering
Fibrin
Muscle Cells
Electric Stimulation
Collagen
Pharmaceutical Preparations
Three Dimensional Printing

Keywords

  • Bioactuator
  • Stereolithography

ASJC Scopus subject areas

  • General

Cite this

Three-dimensionally printed biological machines powered by skeletal muscle. / Cvetkovic, Caroline; Raman, Ritu; Chan, Vincent; Williams, Brian J.; Tolish, Madeline; Bajaj, Piyush; Sakar, Mahmut Selman; Asada, H. Harry; Saif, M. Taher A; Bashir, Rashid.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 28, 15.07.2014, p. 10125-10130.

Research output: Contribution to journalArticle

Cvetkovic, Caroline ; Raman, Ritu ; Chan, Vincent ; Williams, Brian J. ; Tolish, Madeline ; Bajaj, Piyush ; Sakar, Mahmut Selman ; Asada, H. Harry ; Saif, M. Taher A ; Bashir, Rashid. / Three-dimensionally printed biological machines powered by skeletal muscle. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 28. pp. 10125-10130.
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