Three-dimensional imaging of cholesterol and sphingolipids within a madin-darby canine kidney cell

Ashley N. Yeager; Peter K. Weber; Mary L. Kraft

doi:10.1116/1.4939681

Three-dimensional imaging of cholesterol and sphingolipids within a madin-darby canine kidney cell

Ashley N. Yeager, Peter K. Weber, Mary L. Kraft

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Abstract

Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the¹⁸O-cholesterol and¹⁵N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of¹⁸O-cholesterol and¹⁵N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the¹⁸O-enriched regions suggest that these¹⁸O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Biointerphases
Volume	11
Issue number	2
DOIs	https://doi.org/10.1116/1.4939681
State	Published - 2016

ASJC Scopus subject areas

General Chemistry
Biomaterials
General Materials Science
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1116/1.4939681

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title = "Three-dimensional imaging of cholesterol and sphingolipids within a madin-darby canine kidney cell",

abstract = "Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the18O-cholesterol and15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of18O-cholesterol and15N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the18O-enriched regions suggest that these18O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.",

author = "Yeager, {Ashley N.} and Weber, {Peter K.} and Kraft, {Mary L.}",

note = "This work was partially supported by the U.S. NSF under CHE-1508662 (to M.L.K.), and Lab Directed Research and Development funding (to LLNL). A portion of this work was carried out in the Frederick Seitz Materials Research Laboratory Central Research Facilities, University of Illinois. The authors thank Kaiyan Lou for the synthesis of the 15N-sphingolipid precursors and 18O-cholesterol. Work at LLNL was supported by Lab Directed Research and Development funding and performed under the auspices of the U.S. DOE under Contract No. DE-AC52-07NA27344.",

year = "2016",

doi = "10.1116/1.4939681",

language = "English (US)",

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N1 - This work was partially supported by the U.S. NSF under CHE-1508662 (to M.L.K.), and Lab Directed Research and Development funding (to LLNL). A portion of this work was carried out in the Frederick Seitz Materials Research Laboratory Central Research Facilities, University of Illinois. The authors thank Kaiyan Lou for the synthesis of the 15N-sphingolipid precursors and 18O-cholesterol. Work at LLNL was supported by Lab Directed Research and Development funding and performed under the auspices of the U.S. DOE under Contract No. DE-AC52-07NA27344.

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N2 - Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the18O-cholesterol and15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of18O-cholesterol and15N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the18O-enriched regions suggest that these18O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.

AB - Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the18O-cholesterol and15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of18O-cholesterol and15N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the18O-enriched regions suggest that these18O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.

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Three-dimensional imaging of cholesterol and sphingolipids within a madin-darby canine kidney cell

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