Three-dimensional imaging of cholesterol and sphingolipids within a madin-darby canine kidney cell

Ashley N. Yeager, Peter K. Weber, Mary L. Kraft

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the18O-cholesterol and15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of18O-cholesterol and15N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the18O-enriched regions suggest that these18O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiointerphases
Volume11
Issue number2
DOIs
StatePublished - 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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