Abstract
Metabolic stable isotope incorporation and secondary ion mass spectrometry (SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the18O-cholesterol and15N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of18O-cholesterol and15N-sphingolipid enrichment that seem to be separate subcellular compartments. The low levels of nitrogen-containing secondary ions detected at the18O-enriched regions suggest that these18O-cholesterol-rich structures may be lipid droplets, which have a core consisting of cholesterol esters and triacylglycerides.
Original language | English (US) |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Biointerphases |
Volume | 11 |
Issue number | 2 |
DOIs | |
State | Published - 2016 |
ASJC Scopus subject areas
- General Chemistry
- Biomaterials
- General Materials Science
- General Biochemistry, Genetics and Molecular Biology
- General Physics and Astronomy