Third-harmonic generation imaging of breast tissue biopsies

Woowon Lee, Mohammad M. Kabir, Rajyasree Emmadi, Kimani C. Toussaint

Research output: Contribution to journalArticlepeer-review


We demonstrate for the first time the imaging of unstained breast tissue biopsies using third-harmonic generation (THG) microscopy. As a label-free imaging technique, THG microscopy is compared to phase contrast and polarized light microscopy which are standard imaging methods for breast tissues. A simple feature detection algorithm is applied to detect tumour-associated lymphocyte rich regions in unstained breast biopsy tissue and compared with corresponding regions identified by a pathologist from bright-field images of hematoxylin and eosin stained breast tissue. Our results suggest that THG imaging holds potential as a complementary technique for analysing breast tissue biopsies.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalJournal of Microscopy
Issue number2
StatePublished - Nov 1 2016


  • Breast cancer
  • breast tissue biopsy
  • nonlinear microscopy
  • third-harmonic generation
  • tissue microarrays
  • tumour-associated lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology


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