TY - JOUR
T1 - The Xis2d protein of CTnDOT binds to the intergenic region between the mob and tra operons
AU - Hopp, Crystal M.
AU - Gardner, Jeffrey F.
AU - Salyers, Abigail A.
N1 - Funding Information:
We would like to thank Margaret Wood for technical instruction and assistance. We would also like to thank Carolyn Keeton for helpful comments and discussion. This work was funded by the National Institutes of Health , grants AI 22383 and GM 28717 .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - CTnDOT is a 65 kbp integrative and conjugative element (ICE) that carries genes encoding both tetracycline and erythromycin resistances. The excision operon of this element encodes Xis2c, Xis2d, and Exc proteins involved in the excision of CTnDOT from host chromosomes. These proteins are also required in the complex transcriptional regulation of the divergently transcribed transfer (tra) and mobilization (mob) operons of CTnDOT. Transcription of the tra operon is positively regulated by Xis2c and Xis2d, whereas, transcription of the mob operon is positively regulated by Xis2d and Exc. Xis2d is the only protein that is involved in the excision reaction, as well as the transcriptional regulation of both the mob and tra operons. This paper helps establish how Xis2d binds the DNA in the mob and tra region. Unlike other excisionase proteins, Xis2d binds a region of dyad symmetry. The binding site is located in the intergenic region between the mob and tra promoters, and once bound Xis2d induces a bend in the DNA. Xis2d binding to this region could be the preliminary step for the activation of both operons. Then the other proteins, like Exc, can interact with Xis2d and form higher order complexes.
AB - CTnDOT is a 65 kbp integrative and conjugative element (ICE) that carries genes encoding both tetracycline and erythromycin resistances. The excision operon of this element encodes Xis2c, Xis2d, and Exc proteins involved in the excision of CTnDOT from host chromosomes. These proteins are also required in the complex transcriptional regulation of the divergently transcribed transfer (tra) and mobilization (mob) operons of CTnDOT. Transcription of the tra operon is positively regulated by Xis2c and Xis2d, whereas, transcription of the mob operon is positively regulated by Xis2d and Exc. Xis2d is the only protein that is involved in the excision reaction, as well as the transcriptional regulation of both the mob and tra operons. This paper helps establish how Xis2d binds the DNA in the mob and tra region. Unlike other excisionase proteins, Xis2d binds a region of dyad symmetry. The binding site is located in the intergenic region between the mob and tra promoters, and once bound Xis2d induces a bend in the DNA. Xis2d binding to this region could be the preliminary step for the activation of both operons. Then the other proteins, like Exc, can interact with Xis2d and form higher order complexes.
KW - Conjugative transposon
KW - Integrative and conjugative element
KW - Recombination directionality factor
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U2 - 10.1016/j.plasmid.2015.07.002
DO - 10.1016/j.plasmid.2015.07.002
M3 - Article
C2 - 26212728
AN - SCOPUS:84938630725
SN - 0147-619X
VL - 81
SP - 63
EP - 71
JO - Plasmid
JF - Plasmid
ER -