Abstract
Almost two-hundred random sequence decamer-primers were used to screen a pair of bulked samples and the donor parent Malus floribunda clone 821 for markers linked to the V(f) gene conferring resistance to apple scab (Venturia inaequalis (Cke.) Wint.). A single primer was identified which generated a PCR fragment, OPK16/1300, from the donor parent M. floribunda clone 821 and the scab-resistant selections/cultivars bulk, but not from the scab-susceptible recurrent parent bulk. Co-segregation analysis using a segregating apple progeny and polymorphism analysis of individual scab-resistant Coop selections/cultivars confirmed that this marker was linked to the scab-resistance gene V(f) with a recombination frequency of 4.3%. OPK16/1300 was then cloned and sequenced. Sequence-specific primers of 25 oligonucleotides based on the marker were synthesized, and used in turn to screen M. floribunda clone 821, scab-susceptible apple cultivars, scab-resistant apple cultivars, and scab-resistant Coop selections. A pair of sequence-specific primers of clone OPK16/1300 amplified a distinct single band of the same size as the RAPD clone. Thus, a sequence characterized amplified region (SCAR) marker was developed which can be used to identify polymorphisms of OPK16/1300 based on the presence or absence of a single band.
Original language | English (US) |
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Pages (from-to) | 175-182 |
Number of pages | 8 |
Journal | Euphytica |
Volume | 94 |
Issue number | 2 |
DOIs | |
State | Published - 1997 |
Keywords
- Malus
- RAPDs
- SCAR
- V(f) gene
- Venturia inaequalis
- apple
- apple scab resistance
- bulked segregant analysis
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Plant Science
- Horticulture