Abstract
Myosin VI moves toward the pointed (minus) end of actin filaments, the reverse direction of other myosin classes. The myosin VI structure demonstrates that a unique insert at the end of the motor repositions its lever arm and is at least in pact responsible for the reversal of directionality. However, it has been proposed that there must be additional modifications within the motor that contribute to its large step size and to the reversal of directionality. To ascertain the inherent directionality of the motor core, we attached the myosin V lever arm to myosin VI, with and without the unique insect. If the insect was maintained, the motor moved toward the minus end of actin filaments, but if removed, movement was redirected toward the plus end. Single-molecule studies revealed that further adaptations within the motor increase the magnitude and variability of the plus-end directed converter movements, and unexpectedly provide the source of the highly variable myosin VI step size. Thus, the unique insert is necessary and sufficient to reverse an inherently plus-end directed myosin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 778-783 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 104 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 16 2007 |
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Keywords
- Lever arm
- Motility
- Reverse direction
- Unconventional myosin
ASJC Scopus subject areas
- General
Cite this
The unique insert at the end of the myosin VI motor is the sole determinant of directionality. / Park, Hyokeun; Li, Anna; Chen, Li Qiong; Houdusse, Anne; Selvin, Paul R.; Sweeney, H. Lee.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 3, 16.01.2007, p. 778-783.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The unique insert at the end of the myosin VI motor is the sole determinant of directionality
AU - Park, Hyokeun
AU - Li, Anna
AU - Chen, Li Qiong
AU - Houdusse, Anne
AU - Selvin, Paul R.
AU - Sweeney, H. Lee
PY - 2007/1/16
Y1 - 2007/1/16
N2 - Myosin VI moves toward the pointed (minus) end of actin filaments, the reverse direction of other myosin classes. The myosin VI structure demonstrates that a unique insert at the end of the motor repositions its lever arm and is at least in pact responsible for the reversal of directionality. However, it has been proposed that there must be additional modifications within the motor that contribute to its large step size and to the reversal of directionality. To ascertain the inherent directionality of the motor core, we attached the myosin V lever arm to myosin VI, with and without the unique insect. If the insect was maintained, the motor moved toward the minus end of actin filaments, but if removed, movement was redirected toward the plus end. Single-molecule studies revealed that further adaptations within the motor increase the magnitude and variability of the plus-end directed converter movements, and unexpectedly provide the source of the highly variable myosin VI step size. Thus, the unique insert is necessary and sufficient to reverse an inherently plus-end directed myosin.
AB - Myosin VI moves toward the pointed (minus) end of actin filaments, the reverse direction of other myosin classes. The myosin VI structure demonstrates that a unique insert at the end of the motor repositions its lever arm and is at least in pact responsible for the reversal of directionality. However, it has been proposed that there must be additional modifications within the motor that contribute to its large step size and to the reversal of directionality. To ascertain the inherent directionality of the motor core, we attached the myosin V lever arm to myosin VI, with and without the unique insect. If the insect was maintained, the motor moved toward the minus end of actin filaments, but if removed, movement was redirected toward the plus end. Single-molecule studies revealed that further adaptations within the motor increase the magnitude and variability of the plus-end directed converter movements, and unexpectedly provide the source of the highly variable myosin VI step size. Thus, the unique insert is necessary and sufficient to reverse an inherently plus-end directed myosin.
KW - Lever arm
KW - Motility
KW - Reverse direction
KW - Unconventional myosin
UR - http://www.scopus.com/inward/record.url?scp=33846530168&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846530168&partnerID=8YFLogxK
U2 - 10.1073/pnas.0610066104
DO - 10.1073/pnas.0610066104
M3 - Article
C2 - 17213313
AN - SCOPUS:33846530168
VL - 104
SP - 778
EP - 783
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 3
ER -