TY - JOUR
T1 - The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences
AU - Alam, Mohammad Tauqeer
AU - Bora, Hema
AU - Das, Manoj K.
AU - Sharma, Yagya D.
N1 - Funding Information:
Acknowledgements We thank the late Dr. M. A. Ansari for providing laboratory-colonized specimens of the A. stephensi. MTA (Senior Research Fellowship) and HB (Junior Research Fellowship) were supported by Council of Scientific and Industrial Research and Indian Council of Medical Research (ICMR), respectively. We are also grateful to bioinformatics facility of Biotechnology Information System. The experiments conducted in this study comply with the current laws of India. Financial supports for the work were granted by the Department of Biotechnology and ICMR, government of India.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/6
Y1 - 2008/6
N2 - Anopheles (Cellia) stephensi Liston 1901 is one of the major malaria vectors in the Indian subcontinent, Iran, and the Middle East. Three races in this species, namely A. stephensi stephensi (type form), A. stephensi variety mysorensis, and A. stephensi intermediate form, have earlier been reported by several investigators. We describe here the sequencing of the ribosomal DNA internal transcribed spacer 2 (ITS2) and domain-3 (D3) loci of the A. stephensi type and variety mysorensis forms. We also sequenced field-collected adult specimens of this mosquito from three different regions of India. Both forms of A. stephensi showed identical ITS2 and D3 sequences. We did not find any intraspecies sequence variation among the 70 specimens sequenced in this study. In contrast to the eight ITS2 haplotypes observed among Iranian A. stephensi population, we found only one ITS2 haplotype in India. This is the first time to our knowledge that the sequence of the D3 locus of A. stephensi is being reported here. In conclusion, the type and variety mysorensis forms of A. stephensi exhibit identical nucleotide sequences at their ITS2 and D3 loci.
AB - Anopheles (Cellia) stephensi Liston 1901 is one of the major malaria vectors in the Indian subcontinent, Iran, and the Middle East. Three races in this species, namely A. stephensi stephensi (type form), A. stephensi variety mysorensis, and A. stephensi intermediate form, have earlier been reported by several investigators. We describe here the sequencing of the ribosomal DNA internal transcribed spacer 2 (ITS2) and domain-3 (D3) loci of the A. stephensi type and variety mysorensis forms. We also sequenced field-collected adult specimens of this mosquito from three different regions of India. Both forms of A. stephensi showed identical ITS2 and D3 sequences. We did not find any intraspecies sequence variation among the 70 specimens sequenced in this study. In contrast to the eight ITS2 haplotypes observed among Iranian A. stephensi population, we found only one ITS2 haplotype in India. This is the first time to our knowledge that the sequence of the D3 locus of A. stephensi is being reported here. In conclusion, the type and variety mysorensis forms of A. stephensi exhibit identical nucleotide sequences at their ITS2 and D3 loci.
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U2 - 10.1007/s00436-008-0930-7
DO - 10.1007/s00436-008-0930-7
M3 - Article
C2 - 18309520
AN - SCOPUS:43049148288
SN - 0932-0113
VL - 103
SP - 75
EP - 80
JO - Parasitology Research
JF - Parasitology Research
IS - 1
ER -