The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences

Mohammad Tauqeer Alam, Hema Bora, Manoj K. Das, Yagya D. Sharma

Research output: Contribution to journalArticlepeer-review

Abstract

Anopheles (Cellia) stephensi Liston 1901 is one of the major malaria vectors in the Indian subcontinent, Iran, and the Middle East. Three races in this species, namely A. stephensi stephensi (type form), A. stephensi variety mysorensis, and A. stephensi intermediate form, have earlier been reported by several investigators. We describe here the sequencing of the ribosomal DNA internal transcribed spacer 2 (ITS2) and domain-3 (D3) loci of the A. stephensi type and variety mysorensis forms. We also sequenced field-collected adult specimens of this mosquito from three different regions of India. Both forms of A. stephensi showed identical ITS2 and D3 sequences. We did not find any intraspecies sequence variation among the 70 specimens sequenced in this study. In contrast to the eight ITS2 haplotypes observed among Iranian A. stephensi population, we found only one ITS2 haplotype in India. This is the first time to our knowledge that the sequence of the D3 locus of A. stephensi is being reported here. In conclusion, the type and variety mysorensis forms of A. stephensi exhibit identical nucleotide sequences at their ITS2 and D3 loci.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalParasitology Research
Volume103
Issue number1
DOIs
StatePublished - Jun 2008
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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