The two terminal oxidases of the aerobic respiratory chain of Escherichia coli each yield water and not peroxide as a final product

Kimberly Carter Minghetti, Robert B. Gennis

Research output: Contribution to journalArticle

Abstract

The aerobic respiratory chain of Escherichia coli contains two terminal oxidases, the cytochrome d complex and the cytochrome o complex. Each of these enzymes catalyzes the oxidation of ubiquinol-8 within the cytoplasmic membrane and reduces molecular oxygen. The purpose of this work is to experimentally verify that each of the terminal oxidases yields water as a product with no significant amount of hydrogen peroxide. This was accomplished by preparing membranes which were washed so as to eliminate membrane-associated catalase and peroxidase activities. The NADH oxidase activity of the membrane-bound respiratory chain was measured by monitoring the rates of both NADH and oxygen utilization. This was performed using membranes from strains in which either cytochrome o or cytochrome d were absent. Results using each strain showed two NADH utilized per oxygen, indicating a four-electron reduction of oxygen to water.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number1
DOIs
StatePublished - Aug 30 1988

Fingerprint

Peroxides
Electron Transport
Escherichia coli
Cytochrome d Group
Oxidoreductases
Oxygen
Membranes
Water
Cytochromes
NAD
Photosystem II Protein Complex
Catalase
Molecular oxygen
Hydrogen Peroxide
Peroxidase
Cell Membrane
Electrons
Enzymes
Oxidation
Monitoring

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{3764ae2a6ff748d3b6391369dad5f189,
title = "The two terminal oxidases of the aerobic respiratory chain of Escherichia coli each yield water and not peroxide as a final product",
abstract = "The aerobic respiratory chain of Escherichia coli contains two terminal oxidases, the cytochrome d complex and the cytochrome o complex. Each of these enzymes catalyzes the oxidation of ubiquinol-8 within the cytoplasmic membrane and reduces molecular oxygen. The purpose of this work is to experimentally verify that each of the terminal oxidases yields water as a product with no significant amount of hydrogen peroxide. This was accomplished by preparing membranes which were washed so as to eliminate membrane-associated catalase and peroxidase activities. The NADH oxidase activity of the membrane-bound respiratory chain was measured by monitoring the rates of both NADH and oxygen utilization. This was performed using membranes from strains in which either cytochrome o or cytochrome d were absent. Results using each strain showed two NADH utilized per oxygen, indicating a four-electron reduction of oxygen to water.",
author = "{Carter Minghetti}, Kimberly and Gennis, {Robert B.}",
year = "1988",
month = "8",
day = "30",
doi = "10.1016/S0006-291X(88)81075-1",
language = "English (US)",
volume = "155",
pages = "243--248",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The two terminal oxidases of the aerobic respiratory chain of Escherichia coli each yield water and not peroxide as a final product

AU - Carter Minghetti, Kimberly

AU - Gennis, Robert B.

PY - 1988/8/30

Y1 - 1988/8/30

N2 - The aerobic respiratory chain of Escherichia coli contains two terminal oxidases, the cytochrome d complex and the cytochrome o complex. Each of these enzymes catalyzes the oxidation of ubiquinol-8 within the cytoplasmic membrane and reduces molecular oxygen. The purpose of this work is to experimentally verify that each of the terminal oxidases yields water as a product with no significant amount of hydrogen peroxide. This was accomplished by preparing membranes which were washed so as to eliminate membrane-associated catalase and peroxidase activities. The NADH oxidase activity of the membrane-bound respiratory chain was measured by monitoring the rates of both NADH and oxygen utilization. This was performed using membranes from strains in which either cytochrome o or cytochrome d were absent. Results using each strain showed two NADH utilized per oxygen, indicating a four-electron reduction of oxygen to water.

AB - The aerobic respiratory chain of Escherichia coli contains two terminal oxidases, the cytochrome d complex and the cytochrome o complex. Each of these enzymes catalyzes the oxidation of ubiquinol-8 within the cytoplasmic membrane and reduces molecular oxygen. The purpose of this work is to experimentally verify that each of the terminal oxidases yields water as a product with no significant amount of hydrogen peroxide. This was accomplished by preparing membranes which were washed so as to eliminate membrane-associated catalase and peroxidase activities. The NADH oxidase activity of the membrane-bound respiratory chain was measured by monitoring the rates of both NADH and oxygen utilization. This was performed using membranes from strains in which either cytochrome o or cytochrome d were absent. Results using each strain showed two NADH utilized per oxygen, indicating a four-electron reduction of oxygen to water.

UR - http://www.scopus.com/inward/record.url?scp=0023759997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023759997&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(88)81075-1

DO - 10.1016/S0006-291X(88)81075-1

M3 - Article

C2 - 2843179

AN - SCOPUS:0023759997

VL - 155

SP - 243

EP - 248

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -