The sub-cellular distribution of β-catenin in the neural differentiation of ra induced P19 EC cells

Jing Yang, Hai Yu Hou, Wei Bian, Qi Shui Lin, Nai He Jing

Research output: Contribution to journalArticlepeer-review

Abstract

Wnt signals have been shown to play an important role in the development of the central nervous system (CNS) of mouse. In our previous work, it was demonstrated that Wnt signal could initiate differentiation of P19 EC cells. In the present investigation, it was examined with RT-PCR whether expression of β-catenin, a downstream gene of Wnt in its signal transduction pathway, is regulated. It was found that the level of protein or transcript β-catenin during P19 neuronal differentiation was not changed. However, immunostaining data showed that β-catenin was translocalized into nuclei after retinoic acid induced P19 cell aggregates were trypsinized and cultured in serum free N2 medium for 2 and 4 d. In this period, transcription of En-2, a downstream target gene of Wnt signal, increased evidently. The above data suggest that Wnt signals are involved in the early stage of neuronal differentiation process of P19 cell. Meanwhile, the distribution of β-catenin on the neurites indicates that this protein may also be involved in neuritis outgrowth process.

Original languageEnglish (US)
Pages (from-to)671-678
Number of pages8
JournalActa Physiologica Sinica
Volume50
Issue number6
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • Neuronal differentiation
  • P19 EC cells
  • Wnt
  • β-catenin

ASJC Scopus subject areas

  • Medicine(all)

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