The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes

Jennifer B. Rice, Carin K. Vanderpool

Research output: Contribution to journalArticle

Abstract

A number of bacterial small RNAs (sRNAs) act as global regulators of stress responses by controlling expression of multiple genes. The sRNA SgrS is expressed in response to glucose-phosphate stress, a condition associated with disruption of glycolytic flux and accumulation of sugar-phosphates. SgrS has been shown to stimulate degradation of the ptsG mRNA, encoding the major glucose transporter. This study demonstrates that SgrS regulates the genes encoding the mannose and secondary glucose transporter, manXYZ. Analysis of manXYZ mRNA stability and translation in the presence and absence of SgrS indicate that manXYZ is regulated by SgrS under stress conditions and when SgrS is ectopically expressed. In vitro footprinting and in vivo mutational analyses showed that SgrS base pairs with manXYZ within the manX coding sequence to prevent manX translation. Regulation of manX did not require the RNase E degradosome complex, suggesting that the primary mechanism of regulation is translational. An Escherichia coli ptsG mutant strain that is manXYZ+ experiences stress when exposed to the glucose analogs α-methyl glucoside or 2-deoxyglucose. A ptsG manXYZ double mutant is resistant to the stress, indicating that PTS transporters encoded by both SgrS targets are involved in taking up substrates that cause stress.

Original languageEnglish (US)
Pages (from-to)3806-3819
Number of pages14
JournalNucleic acids research
Volume39
Issue number9
DOIs
StatePublished - May 1 2011

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Sugar Phosphates
Facilitative Glucose Transport Proteins
RNA Stability
RNA
Bacterial RNA
Genes
Glucose
Deoxyglucose
Glucosides
Protein Biosynthesis
Mannose
Base Pairing
Phosphates
Escherichia coli
Gene Expression
phosphoenolpyruvate-glucose phosphotransferase

ASJC Scopus subject areas

  • Genetics

Cite this

The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes. / Rice, Jennifer B.; Vanderpool, Carin K.

In: Nucleic acids research, Vol. 39, No. 9, 01.05.2011, p. 3806-3819.

Research output: Contribution to journalArticle

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