The selection of the first AUG as the initiator of eucaryotic mRNAs translation is favored by a 5′-terminal cap group and a purine in the -3′ position

Elzbieta Szczesna-Skorupa, David A. Mead, Byron Kemper

Research output: Contribution to journalArticlepeer-review

Abstract

We have constructed a series of plasmids containing variations in the DNA that codes for the translation initiation region of preproparathyroid hormone. Gel electrophoretic analyses of the character and extent of synthesis of various modified preproparathyroid hormone like proteins derived from the mRNAs transcribed from these plasmids reveal that the presence of a 5′-terminal cap group on mRNA's facilitates recognition of the most 5′-terminal AUG sequence on a mRNA and that AUG sequences within the consensus sequence PuXXAUGPu are favored sites for the initiation of mRNA translation.

Original languageEnglish (US)
Pages (from-to)288-293
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume140
Issue number1
DOIs
StatePublished - Oct 15 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The selection of the first AUG as the initiator of eucaryotic mRNAs translation is favored by a 5′-terminal cap group and a purine in the -3′ position'. Together they form a unique fingerprint.

Cite this