We have constructed a series of plasmids containing variations in the DNA that codes for the translation initiation region of preproparathyroid hormone. Gel electrophoretic analyses of the character and extent of synthesis of various modified preproparathyroid hormone like proteins derived from the mRNAs transcribed from these plasmids reveal that the presence of a 5′-terminal cap group on mRNA's facilitates recognition of the most 5′-terminal AUG sequence on a mRNA and that AUG sequences within the consensus sequence PuXXAUGPu are favored sites for the initiation of mRNA translation.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 15 1986|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology