The secondary structure of ribonuclease P RNA, the catalytic element of a ribonucleoprotein enzyme

Bryan D. James, Gary J. Olsen, Jinsong Liu, Norman R. Pace

Research output: Contribution to journalArticle

Abstract

Secondary structure models for the ribonuclease (RNAase) P RNAs of Bacillus subtilis and E. coll were derived by a phylogenetic comparative analysis of published sequences as well as four novel ones. The RNAase P RNA genes from Bacillus megaterium, Bacillus brevis, Bacillus stearothermophilus, and Pseudomonas fluorescens were cloned, sequenced, and compared with the other available sequences. Regions of pairing were identified by the occurrence of homologous complementary sequences that vary among the compared molecules. A common core of primary and secondary structure can be identified in all these RNAase P RNAs. The previously noted striking differences between the Bacillus and the enteric RNAase P RNAs arise not only from point mutations, but from the addition or deletion of structural domains. The primary and secondary structural features that are common to all of the RNAase P RNAs are likely to be the elements involved in the binding and cleavage of tRNA precursors, and in the interaction with the RNAase P protein.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalCell
Volume52
Issue number1
DOIs
StatePublished - Jan 15 1988

Fingerprint

Ribonuclease P
Catalytic RNA
Ribonucleoproteins
Bacilli
RNA
Enzymes
Bacillus
Bacillus megaterium
Geobacillus stearothermophilus
Pseudomonas fluorescens
RNA Precursors
Ribonucleases
Sequence Homology
Model structures
Bacillus subtilis
Point Mutation
Sequence Analysis
Genes
Molecules
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The secondary structure of ribonuclease P RNA, the catalytic element of a ribonucleoprotein enzyme. / James, Bryan D.; Olsen, Gary J.; Liu, Jinsong; Pace, Norman R.

In: Cell, Vol. 52, No. 1, 15.01.1988, p. 19-26.

Research output: Contribution to journalArticle

James, Bryan D. ; Olsen, Gary J. ; Liu, Jinsong ; Pace, Norman R. / The secondary structure of ribonuclease P RNA, the catalytic element of a ribonucleoprotein enzyme. In: Cell. 1988 ; Vol. 52, No. 1. pp. 19-26.
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