The S-phase-induced lncRNA SUNO1 promotes cell proliferation by controlling YAP1/Hippo signaling pathway

Qinyu Hao, Xinying Zong, Qinyu Sun, Yo-Chuen Lin, You Jin Song, Seyedsasan Hashemikhabir, Rosaline Yc Hsu, Mohammad Kamran, Ritu Chaudhary, Vidisha Tripathi, Deepak Kumar Singh, Arindam Chakraborty, Xiao Ling Li, Yoon Jung Kim, Arturo V Orjalo, Maria Polycarpou-Schwarz, Branden S Moriarity, Lisa M Jenkins, Hans E Johansson, Yuelin J ZhuSven Diederichs, Anindya Bagchi, Tae Hoon Kim, Sarath C Janga, Ashish Lal, Supriya G Prasanth, Kannanganattu V Prasanth

Research output: Contribution to journalArticlepeer-review

Abstract

Cell cycle is a cellular process that is subject to stringent control. In contrast to the wealth of knowledge of proteins controlling the cell cycle, very little is known about the molecular role of lncRNAs (long noncoding RNAs) in cell-cycle progression. By performing genome-wide transcriptome analyses in cell-cycle-synchronized cells, we observed cell-cycle phase-specific induction of >2000 lncRNAs. Further, we demonstrate that an S-phase-upregulated lncRNA, SUNO1, facilitates cell-cycle progression by promoting YAP1-mediated gene expression. SUNO1 facilitates the cell-cycle-specific transcription of WTIP, a positive regulator of YAP1, by promoting the co-activator, DDX5-mediated stabilization of RNA polymerase II on chromatin. Finally, elevated SUNO1 levels are associated with poor cancer prognosis and tumorigenicity, implying its pro-survival role. Thus, we demonstrate the role of a S-phase up-regulated lncRNA in cell-cycle progression via modulating the expression of genes controlling cell proliferation.

Original languageEnglish (US)
JournaleLife
Volume9
DOIs
StatePublished - Oct 27 2020

Keywords

  • cell cycle
  • chromosomes
  • gene expression
  • HCT116
  • human cancer cell lines
  • lncRNA
  • none
  • U2OS
  • WTIP

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)

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