The role of retinoblastoma-associated proteins 46 and 48 in estrogen receptor α mediated gene expression

Amy L. Creekmore, Kjirsten A. Walt, Jennifer R. Schultz-Norton, Yvonne S. Ziegler, Ian X. McLeod, John R. Yates, Ann M. Nardulli

Research output: Contribution to journalArticle

Abstract

The differential recruitment of coregulatory proteins to the DNA-bound estrogen receptor α (ERα) plays a critical role in mediating estrogen-responsive gene expression. We previously isolated and identified retinoblastoma-associated proteins 46 (RbAp46) and 48 (RbAp48), which are associated with chromatin remodeling, histone deacetylation, and transcription repression, as proteins associated with the DNA-bound ERα. We now demonstrate that RbAp46 and RbAp48 interact with ERα in vitro and in vivo, associate with ERα at endogenous, estrogen-responsive genes, and alter expression of endogenous, ERα-activated and -repressed genes in MCF-7 breast cancer cells. Our findings reveal that RbAp48 limits expression of estrogen-responsive genes and that RbAp46 modulates estrogen responsiveness in a gene-specific manner. The ability of RbAp46 and RbAp48 to interact with ERα and influence its activity reveals yet another role for these multifunctional proteins in regulating gene expression.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume291
Issue number1-2
DOIs
StatePublished - Sep 10 2008

Keywords

  • Estrogen receptor
  • RbAp46
  • RbAp48
  • Retinoblastoma-associated protein
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'The role of retinoblastoma-associated proteins 46 and 48 in estrogen receptor α mediated gene expression'. Together they form a unique fingerprint.

  • Cite this