The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing

Yunfang Zhang; Laureen Blahut-Beatty; Suqin Zheng; Steven J. Clough; Daina H. Simmonds

doi:10.1094/MPMI-07-22-0157-R

The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing

Yunfang Zhang, Laureen Blahut-Beatty, Suqin Zheng, Steven J. Clough, Daina H. Simmonds

Research output: Contribution to journal › Article › peer-review

Abstract

The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered trans-genic plants and their progeny were significantly more suscepti-ble to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense.

Original language	English (US)
Pages (from-to)	159-164
Number of pages	6
Journal	Molecular Plant-Microbe Interactions
Volume	36
Issue number	3
DOIs	https://doi.org/10.1094/MPMI-07-22-0157-R
State	Published - Mar 2023

Keywords

14-3-3
CRISPR-Cas9
RNAi
Sclerotinia sclerotiorum
nodulation
plant defense

ASJC Scopus subject areas

Agronomy and Crop Science
Physiology

Online availability

10.1094/MPMI-07-22-0157-R

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title = "The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing",

abstract = "The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered trans-genic plants and their progeny were significantly more suscepti-ble to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense.",

keywords = "14-3-3, CRISPR-Cas9, RNAi, Sclerotinia sclerotiorum, nodulation, plant defense",

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AU - Zhang, Yunfang

AU - Blahut-Beatty, Laureen

AU - Zheng, Suqin

AU - Clough, Steven J.

AU - Simmonds, Daina H.

PY - 2023/3

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N2 - The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered trans-genic plants and their progeny were significantly more suscepti-ble to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense.

AB - The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered trans-genic plants and their progeny were significantly more suscepti-ble to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense.

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The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing

Abstract

Keywords

ASJC Scopus subject areas

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