The RNA component of the Bacillus subtilis RNase P. Sequence, activity, and partial secondary structure

C. Reich, K. J. Gardiner, Gary J Olsen, B. Pace, T. L. Marsh, N. R. Pace

Research output: Contribution to journalArticle

Abstract

The gene defining the catalytic RNA component of RNase P in Bacillus subtilis 168 was cloned into bacteriophage λ and plasmid vectors. The nucleotide sequence of the gene and its surroundings was determined from the cloned DNA and by directly sequencing or reverse transcribing the RNase P RNA. The B. subtilis RNase P RNA sequence (400-401 nucleotides) is remarkably different from that of Escherichia coli (377 nucleotides) (Reed, R.E., Baer, M.F., Guerrier-Takada, C., Donis-Keller, H., and Altman, S. (1982) Cell 30, 627-636; Sakamoto, H., Kimura, N., Nagawa, F., and Shimura, Y. (1983) Nucleic Acids Res. 11, 8237-8251). At best the two are less than 50% similar in sequence. To verify that the RNase P RNA gene was analyzed, a modified, putative gene was cloned adjacent to a bacteriophage T7 promoter and various transcripts were tested for RNase P activity. The intact gene transcript, but not fragments, showed full activity. Full catalytic activity was restored upon mixing the fragments. The extensive differences between the B. subtilis and E. coli RNase P RNAs precluded full covariance analysis of secondary structure, but phylogenetically consistent foldings for portions of both molecules could be derived.

Original languageEnglish (US)
Pages (from-to)7888-7893
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number17
StatePublished - Dec 1 1986
Externally publishedYes

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Ribonuclease P
Bacilli
Bacillus subtilis
RNA
Genes
Bacteriophages
Nucleotides
Escherichia coli
Bacteriophage T7
Catalytic RNA
DNA Sequence Analysis
Nucleic Acids
Catalyst activity
Plasmids
Molecules
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The RNA component of the Bacillus subtilis RNase P. Sequence, activity, and partial secondary structure. / Reich, C.; Gardiner, K. J.; Olsen, Gary J; Pace, B.; Marsh, T. L.; Pace, N. R.

In: Journal of Biological Chemistry, Vol. 261, No. 17, 01.12.1986, p. 7888-7893.

Research output: Contribution to journalArticle

Reich, C, Gardiner, KJ, Olsen, GJ, Pace, B, Marsh, TL & Pace, NR 1986, 'The RNA component of the Bacillus subtilis RNase P. Sequence, activity, and partial secondary structure', Journal of Biological Chemistry, vol. 261, no. 17, pp. 7888-7893.
Reich, C. ; Gardiner, K. J. ; Olsen, Gary J ; Pace, B. ; Marsh, T. L. ; Pace, N. R. / The RNA component of the Bacillus subtilis RNase P. Sequence, activity, and partial secondary structure. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 17. pp. 7888-7893.
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