The restriction endonuclease RflFII, isolated from Ruminococcus flavefaciens FD-1, recognizes the sequence 5′-AGTACT-3′, and is inhibited by site-specific adenine methylation

Mark Morrison, Roderick I. Mackie, Bryan A. White

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular studies of the rumen bacterium Ruminococcus flavefaciens are constrained by the lack of stable gene transfer systems. We report here on the characterization of RflFII, a restriction endonuclease isolated from R. flavefaciens FD-1. The enzyme is an isoschizomer of ScaI, and cleavage of the DNA is blunt-ended, between the internal TA dinucleotide sequence of 5′-AGTACT-3′. Chromosomal DNA preparations were used to demonstrate that adenine methylation of DNA within the sequence 5′-GTAC-3′ inhibits both RflFII and the restriction endonucleases RsaI and ScaI. Chromosomal DNA from R. flavefaciens FD-1 is also host modified to protect against cleavage by ScaI.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalFEMS microbiology letters
Volume122
Issue number1-2
DOIs
StatePublished - Sep 15 1994

Keywords

  • Restriction enzyme
  • Rumen bacteria
  • Ruminococcus flavefaciens

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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