The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli

Lai Lai Yap, Myat T. Lin, Hanlin Ouyang, Rimma I. Samoilova, Sergei A. Dikanov, Robert B. Gennis

Research output: Contribution to journalArticle

Abstract

Cytochrome bo3 is the major respiratory oxidase located in the cytoplasmic membrane of Escherichia coli when grown under high oxygen tension. The enzyme catalyzes the 2-electron oxidation of ubiquinol-8 and the 4-electron reduction of dioxygen to water. When solubilized and isolated using dodecylmaltoside, the enzyme contains one equivalent of ubiquinone-8, bound at a high affinity site (QH). The quinone bound at the QH site can form a stable semiquinone, and the amino acid residues which hydrogen bond to the semiquinone have been identified. In the current work, it is shown that the tightly bound ubiquinone-8 at the QH site is not displaced by ubiquinol-1 even during enzyme turnover. Furthermore, the presence of high affinity inhibitors, HQNO and aurachin C1-10, does not displace ubiquinone-8 from the QH site. The data clearly support the existence of a second binding site for ubiquinone, the QL site, which can rapidly exchange with the substrate pool. HQNO is shown to bind to a single site on the enzyme and to prevent formation of the stable ubisemiquinone, though without displacing the bound quinone. Inhibition of the steady state kinetics of the enzyme indicates that aurachin C1-10 may compete for binding with quinol at the QL site while, at the same time, preventing formation of the ubisemiquinone at the QH site. It is suggested that the two quinone binding sites may be adjacent to each other or partially overlap.

Original languageEnglish (US)
Pages (from-to)1924-1932
Number of pages9
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1797
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

Cytochromes
coenzyme Q10
Escherichia coli
Binding Sites
Enzymes
Electrons
Hydroquinones
Oxygen
Ubiquinone
Hydrogen
Hydrogen bonds
Oxidoreductases
Cell Membrane
benzoquinone
ubiquinol oxidase
Membranes
Amino Acids
Oxidation
Kinetics
Water

Keywords

  • E. coli
  • EPR
  • Oxidase
  • Respiration
  • Ubiquinone

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli. / Yap, Lai Lai; Lin, Myat T.; Ouyang, Hanlin; Samoilova, Rimma I.; Dikanov, Sergei A.; Gennis, Robert B.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1797, No. 12, 01.12.2010, p. 1924-1932.

Research output: Contribution to journalArticle

Yap, Lai Lai ; Lin, Myat T. ; Ouyang, Hanlin ; Samoilova, Rimma I. ; Dikanov, Sergei A. ; Gennis, Robert B. / The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli. In: Biochimica et Biophysica Acta - Bioenergetics. 2010 ; Vol. 1797, No. 12. pp. 1924-1932.
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