The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium

Anuradha Janakiraman, James M. Slauch

Research output: Contribution to journalArticle

Abstract

Salmonella typhimurium is an invasive pathogen that causes diseases ranging from mild gastroenteritis to enteric fever. During the infection process, S. typhimurium induces a number of virulence genes required to circumvent host defences and/or acquire nutrients in the host. We have used the in vivo expression technology (IVET) system to select for S. typhimurium genes that are induced after invasion of a murine cultured cell line. We have characterized a putative iron transporter in Salmonella pathogenicity island 1, termed sitABCD. The sitABCD operon is induced under iron-deficient conditions in vitro and is repressed by Fur. This locus is induced in the animal specifically after invasion of the intestinal epithelium. We show that a sit null mutant is significantly attenuated in BALB/c mice, suggesting that SitABCD plays an important role in iron acquisition in the animal.

Original languageEnglish (US)
Pages (from-to)1146-1155
Number of pages10
JournalMolecular Microbiology
Volume35
Issue number5
DOIs
StatePublished - Mar 20 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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