The proto-oncogene C-KIT maps to canid B-chromosomes

Alexander S. Graphodatsky; Anna V. Kukekova; Dmitry V. Yudkin; Vladimir A. Trifonov; Nadezhda V. Vorobieva; Violetta R. Beklemisheva; Polina L. Perelman; Daria A. Graphodatskaya; Lyudmila N. Trut; Fengtang Yang; Malcolm A. Ferguson-Smith; Gregory M. Acland; Gustavo D. Aguirre

doi:10.1007/s10577-005-7474-9

The proto-oncogene C-KIT maps to canid B-chromosomes

Alexander S. Graphodatsky, Anna V. Kukekova, Dmitry V. Yudkin, Vladimir A. Trifonov, Nadezhda V. Vorobieva, Violetta R. Beklemisheva, Polina L. Perelman, Daria A. Graphodatskaya, Lyudmila N. Trut, Fengtang Yang, Malcolm A. Ferguson-Smith, Gregory M. Acland, Gustavo D. Aguirre

Research output: Contribution to journal › Article › peer-review

Abstract

Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron-exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.

Original language	English (US)
Pages (from-to)	113-122
Number of pages	10
Journal	Chromosome Research
Volume	13
Issue number	2
DOIs	https://doi.org/10.1007/s10577-005-7474-9
State	Published - Jan 2005
Externally published	Yes

Keywords

B-chromosome
C-KIT
Canidae
Oncogene

ASJC Scopus subject areas

Genetics

Online availability

10.1007/s10577-005-7474-9

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title = "The proto-oncogene C-KIT maps to canid B-chromosomes",

abstract = "Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron-exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.",

keywords = "B-chromosome, C-KIT, Canidae, Oncogene",

author = "Graphodatsky, {Alexander S.} and Kukekova, {Anna V.} and Yudkin, {Dmitry V.} and Trifonov, {Vladimir A.} and Vorobieva, {Nadezhda V.} and Beklemisheva, {Violetta R.} and Perelman, {Polina L.} and Graphodatskaya, {Daria A.} and Trut, {Lyudmila N.} and Fengtang Yang and Ferguson-Smith, {Malcolm A.} and Acland, {Gregory M.} and Aguirre, {Gustavo D.}",

note = "Funding Information: We thank Patricia O{\textquoteright}Brien for flow-sorting the fox B-chromosomes, Keith Watamura for his help in figure preparation and Jennifer Johnson for technical assistance. This study was funded in part by the MCB, RFBR, DGPAH, EOB research grants (A.S.G), INTAS Grant to A.S.G and M.A.F.-S., a Wellcome grant to M.A.F.-S., a NATO LST.CLG.979216 to G.D.A, L.N.T., A.S.G., G.M.A., A.V.K., NIMH MH069688– 01 (A.V.K., L.N.T., G.D.A., G.M.A.), NEI, EY06855, EY13729, EY13132 The Foundation Fighting Blindness (G.D.A., G.M.A., A.V.K.), and American Border Collie Association/ U.S.B.C.H.A. (G.M.A., A.V.K.).",

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doi = "10.1007/s10577-005-7474-9",

language = "English (US)",

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journal = "Chromosome Research",

issn = "0967-3849",

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T1 - The proto-oncogene C-KIT maps to canid B-chromosomes

AU - Graphodatsky, Alexander S.

AU - Kukekova, Anna V.

AU - Yudkin, Dmitry V.

AU - Trifonov, Vladimir A.

AU - Vorobieva, Nadezhda V.

AU - Beklemisheva, Violetta R.

AU - Perelman, Polina L.

AU - Graphodatskaya, Daria A.

AU - Trut, Lyudmila N.

AU - Yang, Fengtang

AU - Ferguson-Smith, Malcolm A.

AU - Acland, Gregory M.

AU - Aguirre, Gustavo D.

N1 - Funding Information: We thank Patricia O’Brien for flow-sorting the fox B-chromosomes, Keith Watamura for his help in figure preparation and Jennifer Johnson for technical assistance. This study was funded in part by the MCB, RFBR, DGPAH, EOB research grants (A.S.G), INTAS Grant to A.S.G and M.A.F.-S., a Wellcome grant to M.A.F.-S., a NATO LST.CLG.979216 to G.D.A, L.N.T., A.S.G., G.M.A., A.V.K., NIMH MH069688– 01 (A.V.K., L.N.T., G.D.A., G.M.A.), NEI, EY06855, EY13729, EY13132 The Foundation Fighting Blindness (G.D.A., G.M.A., A.V.K.), and American Border Collie Association/ U.S.B.C.H.A. (G.M.A., A.V.K.).

PY - 2005/1

Y1 - 2005/1

N2 - Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron-exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.

AB - Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron-exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.

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KW - Oncogene

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The proto-oncogene C-KIT maps to canid B-chromosomes

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