The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.

S. Ottonello; D. H. Rivier; G. M. Doolittle; L. S. Young; K. U. Sprague

doi:10.1002/j.1460-2075.1987.tb02452.x

The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.

S. Ottonello, D. H. Rivier, G. M. Doolittle, L. S. Young, K. U. Sprague

Research output: Contribution to journal › Article › peer-review

Abstract

We have resolved a previously unidentified factor (TFIIID) that is required for in vitro transcription of polymerase III templates. Our ability to resolve factor D from each of the other components of the transcription machinery (polymerase and transcription factors IIIB and IIIC) allowed us to test the capacity of these separated components to form stable complexes with tRNA genes. We find that none of the individual components binds detectably to tRNA genes, but that certain combinations of transcription factors do bind. Our results show that TFIIID is essential for binding and that formation of a full transcription complex can proceed by either of two different pathways.

Original language	English (US)
Pages (from-to)	1921-1927
Number of pages	7
Journal	The EMBO journal
Volume	6
Issue number	7
DOIs	https://doi.org/10.1002/j.1460-2075.1987.tb02452.x
State	Published - Jul 1987
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1987.tb02452.x

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abstract = "We have resolved a previously unidentified factor (TFIIID) that is required for in vitro transcription of polymerase III templates. Our ability to resolve factor D from each of the other components of the transcription machinery (polymerase and transcription factors IIIB and IIIC) allowed us to test the capacity of these separated components to form stable complexes with tRNA genes. We find that none of the individual components binds detectably to tRNA genes, but that certain combinations of transcription factors do bind. Our results show that TFIIID is essential for binding and that formation of a full transcription complex can proceed by either of two different pathways.",

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AU - Doolittle, G. M.

AU - Young, L. S.

AU - Sprague, K. U.

PY - 1987/7

Y1 - 1987/7

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AB - We have resolved a previously unidentified factor (TFIIID) that is required for in vitro transcription of polymerase III templates. Our ability to resolve factor D from each of the other components of the transcription machinery (polymerase and transcription factors IIIB and IIIC) allowed us to test the capacity of these separated components to form stable complexes with tRNA genes. We find that none of the individual components binds detectably to tRNA genes, but that certain combinations of transcription factors do bind. Our results show that TFIIID is essential for binding and that formation of a full transcription complex can proceed by either of two different pathways.

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The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.

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