The Possible Relation between a Membrane Conformational Change and Photosystem II Dependent Hydrogen Ion Accumulation and Adenosine 5′-Triphosphate Synthesis

Robert T. Giaquinta, Donald R. Ort, Richard A. Dilley

Research output: Contribution to journalArticle

Abstract

Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide ions were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.

Original languageEnglish (US)
Pages (from-to)4392-4396
Number of pages5
JournalBiochemistry
Volume14
Issue number20
DOIs
StatePublished - Oct 1 1975

Fingerprint

Photosystem II Protein Complex
Plastoquinone
Adenosine
Protons
Adenosine Triphosphate
Reducing Agents
Membranes
Electrons
Iodides
Electron Transport
Benzene
Ions
Fluxes
Oxidation
Water
triphosphoric acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Possible Relation between a Membrane Conformational Change and Photosystem II Dependent Hydrogen Ion Accumulation and Adenosine 5′-Triphosphate Synthesis. / Giaquinta, Robert T.; Ort, Donald R.; Dilley, Richard A.

In: Biochemistry, Vol. 14, No. 20, 01.10.1975, p. 4392-4396.

Research output: Contribution to journalArticle

@article{ed7e4d84fc8f4b5992324fb47a3ac308,
title = "The Possible Relation between a Membrane Conformational Change and Photosystem II Dependent Hydrogen Ion Accumulation and Adenosine 5′-Triphosphate Synthesis",
abstract = "Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide ions were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.",
author = "Giaquinta, {Robert T.} and Ort, {Donald R.} and Dilley, {Richard A.}",
year = "1975",
month = "10",
day = "1",
doi = "10.1021/bi00691a008",
language = "English (US)",
volume = "14",
pages = "4392--4396",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "20",

}

TY - JOUR

T1 - The Possible Relation between a Membrane Conformational Change and Photosystem II Dependent Hydrogen Ion Accumulation and Adenosine 5′-Triphosphate Synthesis

AU - Giaquinta, Robert T.

AU - Ort, Donald R.

AU - Dilley, Richard A.

PY - 1975/10/1

Y1 - 1975/10/1

N2 - Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide ions were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.

AB - Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide ions were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.

UR - http://www.scopus.com/inward/record.url?scp=0016818702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016818702&partnerID=8YFLogxK

U2 - 10.1021/bi00691a008

DO - 10.1021/bi00691a008

M3 - Article

C2 - 240400

AN - SCOPUS:0016818702

VL - 14

SP - 4392

EP - 4396

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 20

ER -