The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli

G. N. Green, H. Fang, R. J. Lin, G. Newton, M. Mather, C. D. Georgiou, R. B. Gennis

Research output: Contribution to journalArticle

Abstract

The cytochrome d terminal oxidase complex is one of two terminal oxidases in the aerobic respiratory chain of Escherichia coli. The enzyme is located in the cytoplasmic membrane where it oxidizes ubiquinol-8 in the bilayer and reduces oxygen to water. Enzyme turnover is coupled to the generation of a proton-motive force, resulting in electrogenic translocation across the membrane of one proton per electron passing through the system. The enzyme is an αβ heterodimer containing four hemes. The cyd locus, encoding both subunits, has previously been genetically mapped and cloned. This work describes an insertion and deletion analysis of cyd which indicates the direction of transcription, defines the coding regions, and suggests that cyd is an operon. In addition, the complete DNA sequence of the cyd gene is reported. Two open reading frames, separated by 18 base pairs, encode the two subunits of the oxidase complex. Hydropathy profiles of the deduced protein sequence indicate that subunits I and II are each likely to have multiple transmembrane elements. There are only 10 histidines in both subunits, several of which are likely to serve as heme axial ligands.

Original languageEnglish (US)
Pages (from-to)13138-13143
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number26
StatePublished - Jan 1 1988

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Cytochrome d Group
Escherichia coli
Oxidoreductases
Nucleotides
Heme
Protons
Enzymes
Membranes
Proton-Motive Force
DNA sequences
Transcription
Operon
Electron Transport
Histidine
Base Pairing
Open Reading Frames
Genes
Cell Membrane
Electrons
Oxygen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli. / Green, G. N.; Fang, H.; Lin, R. J.; Newton, G.; Mather, M.; Georgiou, C. D.; Gennis, R. B.

In: Journal of Biological Chemistry, Vol. 263, No. 26, 01.01.1988, p. 13138-13143.

Research output: Contribution to journalArticle

Green, G. N. ; Fang, H. ; Lin, R. J. ; Newton, G. ; Mather, M. ; Georgiou, C. D. ; Gennis, R. B. / The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 26. pp. 13138-13143.
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AU - Georgiou, C. D.

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