The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes

R. H. Alrefai, P. J. Shiel, L. L. Domier, C. J. D'Arcy, P. H. Berger, S. S. Korban

Research output: Contribution to journalArticle

Abstract

A double-stranded cDNA was synthesized from in vitro polyadenylated apple mosaic virus (ApMV) RNA 3 using oligo(dT) and sequence-specific primers, and was cloned into plasmid vectors. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA 4. ApMV RNA 4 was found to contain an open reading frame (ORF) of 666 nucleotides, which was flanked by 5' and 3' non-translated sequences of 55 and 264 nucleotides, respectively. The ORF encoding the coat protein was identified by comparing the predicted amino acid sequence with that obtained from direct protein microsequencing of the native viral coat protein. The ORF encodes a protein with an M(r) of 25 056. The nucleotide sequence of the ApMV coat protein gene showed no similarity to those of alfalfa mosaic virus, tobacco streak virus (TSV), brome mosaic virus or cucumber mosaic virus. The predicted amino acid sequence of the amino-terminal region of the ApMV coat protein is basic, rich in cysteine residues and may contain a zinc finger motif similar to that found in TSV.

Original languageEnglish (US)
Pages (from-to)2847-2850
Number of pages4
JournalJournal of General Virology
Volume75
Issue number10
DOIs
StatePublished - Jan 1 1994

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Bromoviridae
Mosaic Viruses
Capsid Proteins
Malus
Ilarvirus
Open Reading Frames
RNA
Genes
Amino Acid Sequence
Alfalfa mosaic virus
Nucleotides
Complementary DNA
Bromovirus
Cucumovirus
Zinc Fingers
Cysteine
Proteins
Plasmids
Clone Cells

ASJC Scopus subject areas

  • Virology

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The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes. / Alrefai, R. H.; Shiel, P. J.; Domier, L. L.; D'Arcy, C. J.; Berger, P. H.; Korban, S. S.

In: Journal of General Virology, Vol. 75, No. 10, 01.01.1994, p. 2847-2850.

Research output: Contribution to journalArticle

Alrefai, R. H. ; Shiel, P. J. ; Domier, L. L. ; D'Arcy, C. J. ; Berger, P. H. ; Korban, S. S. / The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes. In: Journal of General Virology. 1994 ; Vol. 75, No. 10. pp. 2847-2850.
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AU - Berger, P. H.

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