The non-photochemical reduction of plastoquinone in leaves

Quentin J. Groom, David M. Kramer, Antony R. Crofts, Donald R. Ort

Research output: Contribution to journalArticle

Abstract

Although it is generally assumed that the plastoquinone pool of thylakoid membranes in leaves of higher plants is rapidly oxidized upon darkening, this is often not the case. A multiflash kinetic fluorimeter was used to monitor the redox state of the plastoquinone pool in leaves. It was found that in many species of plants, particularly those using the NAD-malic enzyme C4 system of photosynthesis, the pool actually became more reduced following a light to dark transition. In some Amaranthus species, plastoquinone remained reduced in the dark for several hours. Far red light, which preferentially drives Photosystem I turnover, could effectively oxidize the plastoquinone pool. Plastoquinone was re-reduced in the dark within a few seconds when far red illumination was removed. The underlying mechanism of the dark reduction of the plastoquinone pool is still uncertain but may involve chlororespiratory activity.

Original languageEnglish (US)
Pages (from-to)205-215
Number of pages11
JournalPhotosynthesis research
Volume36
Issue number3
DOIs
StatePublished - Jun 1 1993

Fingerprint

Plastoquinone
Amaranthus
malic enzyme
far-red light
photosystem I
thylakoids
lighting
leaves
photosynthesis
malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+)
kinetics
Fluorometers
Photosystem I Protein Complex
Light
Thylakoids
Plant Leaves
Photosynthesis
Lighting
Oxidation-Reduction
Membranes

Keywords

  • chlororespiration
  • flash fluorescence
  • photosynthesis

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

The non-photochemical reduction of plastoquinone in leaves. / Groom, Quentin J.; Kramer, David M.; Crofts, Antony R.; Ort, Donald R.

In: Photosynthesis research, Vol. 36, No. 3, 01.06.1993, p. 205-215.

Research output: Contribution to journalArticle

Groom, Quentin J. ; Kramer, David M. ; Crofts, Antony R. ; Ort, Donald R. / The non-photochemical reduction of plastoquinone in leaves. In: Photosynthesis research. 1993 ; Vol. 36, No. 3. pp. 205-215.
@article{2ee1140087114de68c479a72adb6ceb0,
title = "The non-photochemical reduction of plastoquinone in leaves",
abstract = "Although it is generally assumed that the plastoquinone pool of thylakoid membranes in leaves of higher plants is rapidly oxidized upon darkening, this is often not the case. A multiflash kinetic fluorimeter was used to monitor the redox state of the plastoquinone pool in leaves. It was found that in many species of plants, particularly those using the NAD-malic enzyme C4 system of photosynthesis, the pool actually became more reduced following a light to dark transition. In some Amaranthus species, plastoquinone remained reduced in the dark for several hours. Far red light, which preferentially drives Photosystem I turnover, could effectively oxidize the plastoquinone pool. Plastoquinone was re-reduced in the dark within a few seconds when far red illumination was removed. The underlying mechanism of the dark reduction of the plastoquinone pool is still uncertain but may involve chlororespiratory activity.",
keywords = "chlororespiration, flash fluorescence, photosynthesis",
author = "Groom, {Quentin J.} and Kramer, {David M.} and Crofts, {Antony R.} and Ort, {Donald R.}",
year = "1993",
month = "6",
day = "1",
doi = "10.1007/BF00033039",
language = "English (US)",
volume = "36",
pages = "205--215",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - The non-photochemical reduction of plastoquinone in leaves

AU - Groom, Quentin J.

AU - Kramer, David M.

AU - Crofts, Antony R.

AU - Ort, Donald R.

PY - 1993/6/1

Y1 - 1993/6/1

N2 - Although it is generally assumed that the plastoquinone pool of thylakoid membranes in leaves of higher plants is rapidly oxidized upon darkening, this is often not the case. A multiflash kinetic fluorimeter was used to monitor the redox state of the plastoquinone pool in leaves. It was found that in many species of plants, particularly those using the NAD-malic enzyme C4 system of photosynthesis, the pool actually became more reduced following a light to dark transition. In some Amaranthus species, plastoquinone remained reduced in the dark for several hours. Far red light, which preferentially drives Photosystem I turnover, could effectively oxidize the plastoquinone pool. Plastoquinone was re-reduced in the dark within a few seconds when far red illumination was removed. The underlying mechanism of the dark reduction of the plastoquinone pool is still uncertain but may involve chlororespiratory activity.

AB - Although it is generally assumed that the plastoquinone pool of thylakoid membranes in leaves of higher plants is rapidly oxidized upon darkening, this is often not the case. A multiflash kinetic fluorimeter was used to monitor the redox state of the plastoquinone pool in leaves. It was found that in many species of plants, particularly those using the NAD-malic enzyme C4 system of photosynthesis, the pool actually became more reduced following a light to dark transition. In some Amaranthus species, plastoquinone remained reduced in the dark for several hours. Far red light, which preferentially drives Photosystem I turnover, could effectively oxidize the plastoquinone pool. Plastoquinone was re-reduced in the dark within a few seconds when far red illumination was removed. The underlying mechanism of the dark reduction of the plastoquinone pool is still uncertain but may involve chlororespiratory activity.

KW - chlororespiration

KW - flash fluorescence

KW - photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=0000064462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000064462&partnerID=8YFLogxK

U2 - 10.1007/BF00033039

DO - 10.1007/BF00033039

M3 - Article

AN - SCOPUS:0000064462

VL - 36

SP - 205

EP - 215

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 3

ER -