The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa

Michael T. Borchers, Nathaniel L. Harris, Scott C. Wesselkamper, Shiping Zhang, Yi Chen, Lisa Young, Gee W. Lau

Research output: Contribution to journalArticle

Abstract

The NKG2D-activating receptor is expressed on cytotoxic lymphocytes and interacts with ligands expressed on the surface of cells stressed by pathogenic and nonpathogenic stimuli. In this study, we investigated the physiologic importance of NKG2D receptor-ligand interactions in response to acute pulmonary Pseudomonas aeruginosa infection. P. aeruginosa infection increased the expression of mouse NKG2D ligands (Rae1) in airway epithelial cells and alveolar macrophages in vivo and also increased the cell surface expression of human NKG2D ligands (ULBP2) on airway epithelial cells in vitro. NKG2D receptor blockade with a specific monoclonal antibody inhibited the pulmonary clearance of P. aeruginosa. NKG2D receptor blockade also resulted in decreased production of Th1 cytokines and nitric oxide in the lungs of P. aeruginosa-infected mice. Additionally, NKG2D receptor blockade reduced the epithelial cell sloughing that accompanies P. aeruginosa infection. Macrophage phagocytosis and bronchoalveolar lavage cellularity were not different in P. aeruglnosa-infected mice with and without NKG2D receptor blockade. These results demonstrate the importance of NKG2D-mediated immune activation in the clearance of acute bacterial infection and suggest that epithelial cell-lymphocyte interactions mediate pulmonary cytokine production, epithelial cell integrity, and bacterial clearance.

Original languageEnglish (US)
Pages (from-to)2578-2586
Number of pages9
JournalInfection and immunity
Volume74
Issue number5
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

NK Cell Lectin-Like Receptor Subfamily K
Pseudomonas aeruginosa
Pseudomonas Infections
Epithelial Cells
Lung
Ligands
Lymphocytes
Cytokines
Alveolar Macrophages
Bronchoalveolar Lavage
Phagocytosis
Bacterial Infections
Cell Communication
Nitric Oxide
Macrophages
Monoclonal Antibodies

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Borchers, M. T., Harris, N. L., Wesselkamper, S. C., Zhang, S., Chen, Y., Young, L., & Lau, G. W. (2006). The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa. Infection and immunity, 74(5), 2578-2586. https://doi.org/10.1128/IAI.74.5.2578-2586.2006

The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa. / Borchers, Michael T.; Harris, Nathaniel L.; Wesselkamper, Scott C.; Zhang, Shiping; Chen, Yi; Young, Lisa; Lau, Gee W.

In: Infection and immunity, Vol. 74, No. 5, 01.05.2006, p. 2578-2586.

Research output: Contribution to journalArticle

Borchers, MT, Harris, NL, Wesselkamper, SC, Zhang, S, Chen, Y, Young, L & Lau, GW 2006, 'The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa', Infection and immunity, vol. 74, no. 5, pp. 2578-2586. https://doi.org/10.1128/IAI.74.5.2578-2586.2006
Borchers MT, Harris NL, Wesselkamper SC, Zhang S, Chen Y, Young L et al. The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa. Infection and immunity. 2006 May 1;74(5):2578-2586. https://doi.org/10.1128/IAI.74.5.2578-2586.2006
Borchers, Michael T. ; Harris, Nathaniel L. ; Wesselkamper, Scott C. ; Zhang, Shiping ; Chen, Yi ; Young, Lisa ; Lau, Gee W. / The NKG2D-activating receptor mediates pulmonary clearance of Pseudomonas aeruginosa. In: Infection and immunity. 2006 ; Vol. 74, No. 5. pp. 2578-2586.
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