The myostatin null mutation and clenbuterol administration elicit additive effects in mice

A. C. Dilger, S. R. Gabriel, L. W. Kutzler, F. K. McKeith, J. Killefer

Research output: Contribution to journalArticlepeer-review

Abstract

In mice, the myostatin (Mstn) null mutation and treatment with clenbuterol both increase muscle growth and decrease fat mass. Our objective was to determine whether mechanistic overlap exists by administering clenbuterol to Mstn null mice. Male Mstn null and wild-type mice of similar genetic backgrounds received either 0 (control) or 20 p.p.m. clenbuterol in tap water free choice for 14 days. Several traits were measured to estimate muscle and fat growth. The Mstn null mutation resulted in increased body and empty carcass weight, increased muscle weights and decreased fat pad weights. Fat content was reduced and protein content was increased in the empty carcasses of Mstn null mice. Similarly, treatment with clenbuterol resulted in increased body and empty carcass weight, increased muscle weights and reduced fat pad weights. Fat content of empty carcasses and viscera was reduced and protein content of empty carcasses was increased with clenbuterol treatment. A significant interaction of genotype and clenbuterol treatment would indicate an altered responsiveness of Mstn null mice to clenbuterol. However, only the weight of gastrocnemius muscles exhibited a significant (P = 0.01) interaction of genotype and clenbuterol treatment, indicating that Mstn null mice were less responsive to clenbuterol compared with wild-type mice. Thus, for all other traits, the impact of Mstn null mutation and clenbuterol treatment was completely additive. These data suggest that disruption of Mstn function does not alter the response of mice to β-adrenergic agonists.

Original languageEnglish (US)
Pages (from-to)466-471
Number of pages6
JournalAnimal
Volume4
Issue number3
DOIs
StatePublished - May 2010

Keywords

  • Clenbuterol
  • Mouse
  • Myostatin
  • β-adrenergic agonist

ASJC Scopus subject areas

  • Animal Science and Zoology

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