The murine genes Hox-5.1 and Hox-4.1 belong to the same HOX complex on chromosome 2

Lisa Stubbs, Annemarie Poustka, Agnès Baron, Hans Lehrach, Peter Lonai, Denis Duboule

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Two different loci of Antennapedia-related homeobox-containing genes have been shown to map to mouse chromosome 2: the HOX-5 complex and the Hox-4.1 gene. These independently derived loci are likely to be parts of a single gene complex, although their close linkage has not yet been demonstrated. Since cosmid walks to extend the HOX-5 cluster and to potentially link the two loci were unsuccessful, we have used large restriction fragments separated by pulsed-field gel electrophoresis to demonstrate the linkage between probes from the HOX-5 region and sequences near Hox-4.1. To further define the distance between the two linked loci, we screened a NotI jumping library with sequences near the Hox-5.1 gene to obtain a marker within the region predicted to contain Hox-4.1. The jumping endpoint lies within genomic clones from a λ phage walk extending from the 5′ end of Hox-4.1, and thus provides clear evidence of linkage between the two Hox loci. Our results demonstrate that Hox-4.1 lies approximately 35 kb downstream of the Hox-5.1 gene and that the two loci do indeed thus constitute parts of the same HOX complex.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
Issue number3
StatePublished - Jul 1990
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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