Abstract
Axonal tension exists in neurons and may be involved in neuronal signaling. We study the effect of mechanical strain on the dynamics of vesicles in in vivo Drosophila motor neurons and in vitro Aplysia neurons. Neurons are stretched or compressed while observing vesicle dynamics by high-resolution live-imaging. In response to mechanical stretch we observe the dynamic accumulation of synaptic vesicles at the in vivo neuromuscular junction (NMJ) after approximately 50 min. Vesicle accumulation at the NMJ persists for at least 30 min after stretch is removed. In response to compression we observe disruption of vesicle dynamics in in vitro growth cones. Range and processivity of vesicle motion decrease immediately after applied compression and do not recover for at least 20 min after compression is removed. Through live-imaging this study shows that mechanical stretch promotes vesicle clustering in in vivo synapses, and compression impedes vesicle transport in in vitro growth cones.
| Original language | English (US) |
|---|---|
| Title of host publication | Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 |
| Pages | 436-439 |
| Number of pages | 4 |
| State | Published - Nov 23 2011 |
| Event | Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 - Boston, MA, United States Duration: Jun 13 2011 → Jun 16 2011 |
Publication series
| Name | Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 |
|---|---|
| Volume | 3 |
Other
| Other | Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 |
|---|---|
| Country | United States |
| City | Boston, MA |
| Period | 6/13/11 → 6/16/11 |
Fingerprint
Keywords
- In vitro
- In vivo
- Mechanical strain
- Neuron
- Vesicle
ASJC Scopus subject areas
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering
Cite this
The mechanical sensitivity of vesicle dynamics in in vivo and in vitro neurons. / Ahmed, W. W.; Li, T. C.; Rubakhin, Stanislav; Chiba, A.; Sweedler, Jonathan V; Saif, M Taher A.
Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011. 2011. p. 436-439 (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011; Vol. 3).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - The mechanical sensitivity of vesicle dynamics in in vivo and in vitro neurons
AU - Ahmed, W. W.
AU - Li, T. C.
AU - Rubakhin, Stanislav
AU - Chiba, A.
AU - Sweedler, Jonathan V
AU - Saif, M Taher A
PY - 2011/11/23
Y1 - 2011/11/23
N2 - Axonal tension exists in neurons and may be involved in neuronal signaling. We study the effect of mechanical strain on the dynamics of vesicles in in vivo Drosophila motor neurons and in vitro Aplysia neurons. Neurons are stretched or compressed while observing vesicle dynamics by high-resolution live-imaging. In response to mechanical stretch we observe the dynamic accumulation of synaptic vesicles at the in vivo neuromuscular junction (NMJ) after approximately 50 min. Vesicle accumulation at the NMJ persists for at least 30 min after stretch is removed. In response to compression we observe disruption of vesicle dynamics in in vitro growth cones. Range and processivity of vesicle motion decrease immediately after applied compression and do not recover for at least 20 min after compression is removed. Through live-imaging this study shows that mechanical stretch promotes vesicle clustering in in vivo synapses, and compression impedes vesicle transport in in vitro growth cones.
AB - Axonal tension exists in neurons and may be involved in neuronal signaling. We study the effect of mechanical strain on the dynamics of vesicles in in vivo Drosophila motor neurons and in vitro Aplysia neurons. Neurons are stretched or compressed while observing vesicle dynamics by high-resolution live-imaging. In response to mechanical stretch we observe the dynamic accumulation of synaptic vesicles at the in vivo neuromuscular junction (NMJ) after approximately 50 min. Vesicle accumulation at the NMJ persists for at least 30 min after stretch is removed. In response to compression we observe disruption of vesicle dynamics in in vitro growth cones. Range and processivity of vesicle motion decrease immediately after applied compression and do not recover for at least 20 min after compression is removed. Through live-imaging this study shows that mechanical stretch promotes vesicle clustering in in vivo synapses, and compression impedes vesicle transport in in vitro growth cones.
KW - In vitro
KW - In vivo
KW - Mechanical strain
KW - Neuron
KW - Vesicle
UR - http://www.scopus.com/inward/record.url?scp=81455132739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81455132739&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:81455132739
SN - 9781439871386
T3 - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
SP - 436
EP - 439
BT - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
ER -