The low noise limit in gene expression

Roy David Dar, Brandon S. Razooky, Leor S. Weinberger, Chris D. Cox, Michael L. Simpson

Research output: Contribution to journalArticle

Abstract

Protein noise measurements are increasingly used to elucidate biophysical parameters. Unfortunately noise analyses are often at odds with directly measured parameters. Here we show that these inconsistencies arise from two problematic analytical choices: (i) the assumption that protein translation rate is invariant for different proteins of different abundances, which has inadvertently led to (ii) the assumption that a large constitutive extrinsic noise sets the low noise limit in gene expression. While growing evidence suggests that transcriptional bursting may set the low noise limit, variability in translational bursting has been largely ignored. We show that genome-wide systematic variation in translational efficiency can-and in the case of E. coli does-control the low noise limit in gene expression. Therefore constitutive extrinsic noise is small and only plays a role in the absence of a systematic variation in translational efficiency. These results show the existence of two distinct expression noise patterns: (1) a global noise floor uniformly imposed on all genes by expression bursting; and (2) high noise distributed to only a select group of genes.

Original languageEnglish (US)
Article numbere0140969
JournalPloS one
Volume10
Issue number10
DOIs
StatePublished - Oct 21 2015

Fingerprint

Gene expression
Noise
Genes
Gene Expression
gene expression
Proteins
Escherichia coli
Protein Biosynthesis
proteins
cans
translation (genetics)
Genome
genes
genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dar, R. D., Razooky, B. S., Weinberger, L. S., Cox, C. D., & Simpson, M. L. (2015). The low noise limit in gene expression. PloS one, 10(10), [e0140969]. https://doi.org/10.1371/journal.pone.0140969

The low noise limit in gene expression. / Dar, Roy David; Razooky, Brandon S.; Weinberger, Leor S.; Cox, Chris D.; Simpson, Michael L.

In: PloS one, Vol. 10, No. 10, e0140969, 21.10.2015.

Research output: Contribution to journalArticle

Dar, RD, Razooky, BS, Weinberger, LS, Cox, CD & Simpson, ML 2015, 'The low noise limit in gene expression', PloS one, vol. 10, no. 10, e0140969. https://doi.org/10.1371/journal.pone.0140969
Dar RD, Razooky BS, Weinberger LS, Cox CD, Simpson ML. The low noise limit in gene expression. PloS one. 2015 Oct 21;10(10). e0140969. https://doi.org/10.1371/journal.pone.0140969
Dar, Roy David ; Razooky, Brandon S. ; Weinberger, Leor S. ; Cox, Chris D. ; Simpson, Michael L. / The low noise limit in gene expression. In: PloS one. 2015 ; Vol. 10, No. 10.
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