The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein

Markus Bachmann; Joan L. Huber; Pao Chi Liao; Douglas A. Gage; Steven C. Huber

doi:10.1016/0014-5793(96)00478-4

The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein

Markus Bachmann, Joan L. Huber, Pao Chi Liao, Douglas A. Gage, Steven C. Huber

Plant Biology

Research output: Contribution to journal › Article

Abstract

The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.

Original language	English (US)
Pages (from-to)	127-131
Number of pages	5
Journal	FEBS Letters
Volume	387
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00478-4
State	Published - Jun 3 1996

Keywords

14-3-3 proteins
Inhibitor
Nitrate reductase
Protein phosphorylation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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abstract = "The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.",

keywords = "14-3-3 proteins, Inhibitor, Nitrate reductase, Protein phosphorylation",

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AU - Huber, Steven C.

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