The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein

Markus Bachmann, Joan L. Huber, Pao Chi Liao, Douglas A. Gage, Steven C. Huber

Research output: Contribution to journalArticle

Abstract

The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.

Original languageEnglish (US)
Pages (from-to)127-131
Number of pages5
JournalFEBS Letters
Volume387
Issue number2-3
DOIs
StatePublished - Jun 3 1996

Fingerprint

14-3-3 Proteins
Nitrate Reductase
Spinacia oleracea
Proteins
Nitrate Reductase (NADH)
Monoclonal Antibodies
Arabidopsis
Magnesium
Amino Acid Sequence
Amino Acids
Peptides

Keywords

  • 14-3-3 proteins
  • Inhibitor
  • Nitrate reductase
  • Protein phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein. / Bachmann, Markus; Huber, Joan L.; Liao, Pao Chi; Gage, Douglas A.; Huber, Steven C.

In: FEBS Letters, Vol. 387, No. 2-3, 03.06.1996, p. 127-131.

Research output: Contribution to journalArticle

Bachmann, Markus ; Huber, Joan L. ; Liao, Pao Chi ; Gage, Douglas A. ; Huber, Steven C. / The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein. In: FEBS Letters. 1996 ; Vol. 387, No. 2-3. pp. 127-131.
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