@article{de2a80f5d8804a21a0b79cd0098226ce,
title = "The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein",
abstract = "The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.",
keywords = "14-3-3 proteins, Inhibitor, Nitrate reductase, Protein phosphorylation",
author = "Markus Bachmann and Huber, {Joan L.} and Liao, {Pao Chi} and Gage, {Douglas A.} and Huber, {Steven C.}",
note = "Funding Information: A {"}knowledgements. This research represents cooperative investiga-ti ,ns of the U.S. Department of Agriculture (USDA), Agricultural R.'search Service, and the North Carolina Agricultural Research S~ rvice. The research in the author's laboratory is supported in part b grants from the U.S. Department of Energy (Grant DE-AI05-9 ER20031 to S.C.H.) and USDA-NRI (Grant 93-37305-9231 to J. ~.H. and S.C.H.). Mass spectral data were acquired at the MSU-Mass Spectrometry Facility, which is supported in part by a grant (1~ R-00480) from the NIH, National Center for Research Resources. V~e thank Dr. Robert J. Ferl for supplying recombinant Arabidopsis G F14t0 protein and the mouse anti-GF14 monoclonal antibody. His ~)rk is supported by a grant from USDA-NRI (Grant 93-37304-9t 08). Mention of a trademark or proprietary product does not con-sl tute a guarantee or warranty of the product by the USDA or the N )rth Carolina Agricultural Research Service and does not imply its atproval to the exclusion of other products that might also be sl itable.",
year = "1996",
month = jun,
day = "3",
doi = "10.1016/0014-5793(96)00478-4",
language = "English (US)",
volume = "387",
pages = "127--131",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",
}