Abstract
The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 127-131 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 387 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jun 3 1996 |
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Keywords
- 14-3-3 proteins
- Inhibitor
- Nitrate reductase
- Protein phosphorylation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein. / Bachmann, Markus; Huber, Joan L.; Liao, Pao Chi; Gage, Douglas A.; Huber, Steven C.
In: FEBS Letters, Vol. 387, No. 2-3, 03.06.1996, p. 127-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein
AU - Bachmann, Markus
AU - Huber, Joan L.
AU - Liao, Pao Chi
AU - Gage, Douglas A.
AU - Huber, Steven C.
PY - 1996/6/3
Y1 - 1996/6/3
N2 - The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.
AB - The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14ω.
KW - 14-3-3 proteins
KW - Inhibitor
KW - Nitrate reductase
KW - Protein phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=0029924885&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029924885&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(96)00478-4
DO - 10.1016/0014-5793(96)00478-4
M3 - Article
C2 - 8674533
AN - SCOPUS:0029924885
VL - 387
SP - 127
EP - 131
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -