The influence of protein and interfacial structure on the self-assembly of oriented protein arrays

Research output: Contribution to journalArticle

Abstract

These results demonstrate the complexity of factors that impact the self-assembly of protein arrays via the specific binding to receptor- functionalized interfaces. Both the composition and colloidal properties of the protein and target membrane surfaces will affect the protein-surface interactions. However, different structural features control the interactions over different distance regimes and with different consequences. The long- range interactions that control the adsorption kinetics are sensitive not only to the charge on the target surface but also by the topological charge distribution on the protein exterior. Short-range repulsive interactions rooted in both the protein topology and in the membrane structure, by contrast, can significantly alter both the rates and strengths binding. Consequently, the effective design of self-assembling protein arrays must consider not only the recognition interactions that drive such self- organization, but also the details of the micro-environment and their impact on molecular recognition events.

Original languageEnglish (US)
Pages (from-to)173-190
Number of pages18
JournalAdvances in Biophysics
Volume34
DOIs
StatePublished - Jan 1 1997

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Protein Array Analysis
Self assembly
Membrane Proteins
Adsorption
Proteins
Membranes
Molecular recognition
Membrane structures
Charge distribution
Topology
Kinetics
Chemical analysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

The influence of protein and interfacial structure on the self-assembly of oriented protein arrays. / Leckband, Deborah E.

In: Advances in Biophysics, Vol. 34, 01.01.1997, p. 173-190.

Research output: Contribution to journalArticle

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