The influence of cytokinins and ionic strength of Anderson's medium on shoot establishment and proliferation of evergreen azalea

Shoot-tip explants of evergreen azalea cv. Fuchsia grown on Anderson's medium and containing different cytokinins produced the highest proliferation rate on a medium containing thidiazuron (TDZ). TDZ concentrations ranging from 0.23 to 2.3 μM resulted in both good bud-break rate (4 to 5) and shoot quality (> 0.5 cm in length). Adding 2.3 μM zeatin to Anderson's medium containing 0.23 or 2.3 μM TDZ increased the number of axillary shoots/explant. However, increasing the zeatin concentration to 4.6 μM resulted in a reduced shoot proliferation rate. A medium containing 1.15 μM TDZ and 2.3 μM zeatin resulted in an 18-fold increase for 'Fuchsia' and a 9-fold increase for 'Hino Crimson' after 6 weeks of culture. It was found that explants grown on a half-strength Anderson's medium with 87.6 mM sucrose generally had better shoot proliferation rate and shoot quality than at higher ionic strength.