TY - JOUR
T1 - The impact of mechanically stimulated muscle-derived stromal cells on aged skeletal muscle
AU - Huntsman, Heather D.
AU - Rendeiro, Catarina
AU - Merritt, Jennifer R.
AU - Pincu, Yair
AU - Cobert, Adam
AU - De Lisio, Michael
AU - Kolyvas, Emily
AU - Dvoretskiy, Svyatoslav
AU - Dobrucki, Iwona T.
AU - Kemkemer, Ralf
AU - Jensen, Tor
AU - Dobrucki, Lawrence W.
AU - Rhodes, Justin S.
AU - Boppart, Marni D.
N1 - Funding Information:
HDH was funded at UIUC from National Science Foundation (NSF) Grant 0965918 IGERT: Training the Next Generation of Researchers in Cellular and Molecular Mechanics and BioNanotechnology. This work was supported by grants from the Ellison Medical Foundation ( AG-NS-0547-09 ) (to MDB) and Abbott Nutrition through the Center for Nutrition, Learning, and Memory (CNLM) at the University of Illinois (CNLM ZA68 to MDB and ZB32 to JSR).
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/3
Y1 - 2018/3
N2 - Perivascular stromal cells, including mesenchymal stem/stromal cells (MSCs), secrete paracrine factor in response to exercise training that can facilitate improvements in muscle remodeling. This study was designed to test the capacity for muscle-resident MSCs (mMSCs) isolated from young mice to release regenerative proteins in response to mechanical strain in vitro, and subsequently determine the extent to which strain-stimulated mMSCs can enhance skeletal muscle and cognitive performance in a mouse model of uncomplicated aging. Protein arrays confirmed a robust increase in protein release at 24 h following an acute bout of mechanical strain in vitro (10%, 1 Hz, 5 h) compared to non-strain controls. Aged (24 month old), C57BL/6 mice were provided bilateral intramuscular injection of saline, non-strain control mMSCs, or mMSCs subjected to a single bout of mechanical strain in vitro (4 × 10 4 ). No significant changes were observed in muscle weight, myofiber size, maximal force, or satellite cell quantity at 1 or 4 wks between groups. Peripheral perfusion was significantly increased in muscle at 4 wks post-mMSC injection (p < 0.05), yet no difference was noted between control and preconditioned mMSCs. Intramuscular injection of preconditioned mMSCs increased the number of new neurons and astrocytes in the dentate gyrus of the hippocampus compared to both control groups (p < 0.05), with a trend toward an increase in water maze performance noted (p = 0.07). Results from this study demonstrate that acute injection of exogenously stimulated muscle-resident stromal cells do not robustly impact aged muscle structure and function, yet increase the survival of new neurons in the hippocampus.
AB - Perivascular stromal cells, including mesenchymal stem/stromal cells (MSCs), secrete paracrine factor in response to exercise training that can facilitate improvements in muscle remodeling. This study was designed to test the capacity for muscle-resident MSCs (mMSCs) isolated from young mice to release regenerative proteins in response to mechanical strain in vitro, and subsequently determine the extent to which strain-stimulated mMSCs can enhance skeletal muscle and cognitive performance in a mouse model of uncomplicated aging. Protein arrays confirmed a robust increase in protein release at 24 h following an acute bout of mechanical strain in vitro (10%, 1 Hz, 5 h) compared to non-strain controls. Aged (24 month old), C57BL/6 mice were provided bilateral intramuscular injection of saline, non-strain control mMSCs, or mMSCs subjected to a single bout of mechanical strain in vitro (4 × 10 4 ). No significant changes were observed in muscle weight, myofiber size, maximal force, or satellite cell quantity at 1 or 4 wks between groups. Peripheral perfusion was significantly increased in muscle at 4 wks post-mMSC injection (p < 0.05), yet no difference was noted between control and preconditioned mMSCs. Intramuscular injection of preconditioned mMSCs increased the number of new neurons and astrocytes in the dentate gyrus of the hippocampus compared to both control groups (p < 0.05), with a trend toward an increase in water maze performance noted (p = 0.07). Results from this study demonstrate that acute injection of exogenously stimulated muscle-resident stromal cells do not robustly impact aged muscle structure and function, yet increase the survival of new neurons in the hippocampus.
KW - Aging
KW - Cognition
KW - Exercise
KW - Mesenchymal stem cells
KW - Neurogenesis
KW - Perivascular stromal cells
KW - Skeletal muscle
KW - Vascular perfusion
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U2 - 10.1016/j.exger.2017.12.012
DO - 10.1016/j.exger.2017.12.012
M3 - Article
C2 - 29269268
AN - SCOPUS:85039704972
VL - 103
SP - 35
EP - 46
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
ER -