The impact of aging, hearing loss, and body weight on mouse hippocampal redox state, measured in brain slices using fluorescence imaging

Kevin A. Stebbings, Hyun W. Choi, Aditya Ravindra, Daniel Adolfo Llano

Research output: Contribution to journalArticlepeer-review

Abstract

The relationships between oxidative stress in the hippocampus and other aging-related changes such as hearing loss, cortical thinning, or changes in body weight are not yet known. We measured the redox ratio in a number of neural structures in brain slices taken from young and aged mice. Hearing thresholds, body weight, and cortical thickness were also measured. We found striking aging-related increases in the redox ratio that were isolated to the stratum pyramidale, while such changes were not observed in thalamus or cortex. These changes were driven primarily by changes in flavin adenine dinucleotide, not nicotinamide adenine dinucleotide hydride. Multiple regression analysis suggested that neither hearing threshold nor cortical thickness independently contributed to this change in hippocampal redox ratio. However, body weight did independently contribute to predicted changes in hippocampal redox ratio. These data suggest that aging-related changes in hippocampal redox ratio are not a general reflection of overall brain oxidative state but are highly localized, while still being related to at least one marker of late aging, weight loss at the end of life.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalNeurobiology of Aging
Volume42
DOIs
StatePublished - Jun 1 2016

Keywords

  • Auditory
  • Cortex
  • Flavoprotein
  • Hippocampus
  • Imaging
  • Presbycusis
  • Redox

ASJC Scopus subject areas

  • General Neuroscience
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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