The hydrodynamic motion of Nanodiscs

Tyler Camp, Mark McLean, Mallory Kato, Lionel Cheruzel, Stephen Sligar

Research output: Contribution to journalArticle

Abstract

We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalChemistry and Physics of Lipids
Volume220
DOIs
StatePublished - May 2019

Keywords

  • Nanodiscs
  • Rotational motion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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