The hydrodynamic motion of Nanodiscs

Tyler Camp, Mark McLean, Mallory Kato, Lionel Cheruzel, Stephen Sligar

Research output: Contribution to journalArticle

Abstract

We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalChemistry and Physics of Lipids
Volume220
DOIs
StatePublished - May 2019

Fingerprint

Hydrodynamics
Membrane Proteins
Lipid bilayers
Lipid Bilayers
Membranes
Scaffolds
Labels
Proteins
Ruthenium
Fluorescence
Lipids
Monitoring

Keywords

  • Nanodiscs
  • Rotational motion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

Cite this

The hydrodynamic motion of Nanodiscs. / Camp, Tyler; McLean, Mark; Kato, Mallory; Cheruzel, Lionel; Sligar, Stephen.

In: Chemistry and Physics of Lipids, Vol. 220, 05.2019, p. 28-35.

Research output: Contribution to journalArticle

Camp, Tyler ; McLean, Mark ; Kato, Mallory ; Cheruzel, Lionel ; Sligar, Stephen. / The hydrodynamic motion of Nanodiscs. In: Chemistry and Physics of Lipids. 2019 ; Vol. 220. pp. 28-35.
@article{d920c1b32ecc4a0da23ac0f568dcf4b4,
title = "The hydrodynamic motion of Nanodiscs",
abstract = "We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.",
keywords = "Nanodiscs, Rotational motion",
author = "Tyler Camp and Mark McLean and Mallory Kato and Lionel Cheruzel and Stephen Sligar",
year = "2019",
month = "5",
doi = "10.1016/j.chemphyslip.2019.02.008",
language = "English (US)",
volume = "220",
pages = "28--35",
journal = "Chemistry and Physics of Lipids",
issn = "0009-3084",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - The hydrodynamic motion of Nanodiscs

AU - Camp, Tyler

AU - McLean, Mark

AU - Kato, Mallory

AU - Cheruzel, Lionel

AU - Sligar, Stephen

PY - 2019/5

Y1 - 2019/5

N2 - We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.

AB - We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.

KW - Nanodiscs

KW - Rotational motion

UR - http://www.scopus.com/inward/record.url?scp=85062224380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062224380&partnerID=8YFLogxK

U2 - 10.1016/j.chemphyslip.2019.02.008

DO - 10.1016/j.chemphyslip.2019.02.008

M3 - Article

C2 - 30802435

AN - SCOPUS:85062224380

VL - 220

SP - 28

EP - 35

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

SN - 0009-3084

ER -