Abstract
We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 28-35 |
| Number of pages | 8 |
| Journal | Chemistry and Physics of Lipids |
| Volume | 220 |
| DOIs | |
| State | Published - May 2019 |
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Keywords
- Nanodiscs
- Rotational motion
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Cell Biology
Cite this
The hydrodynamic motion of Nanodiscs. / Camp, Tyler; McLean, Mark; Kato, Mallory; Cheruzel, Lionel; Sligar, Stephen.
In: Chemistry and Physics of Lipids, Vol. 220, 05.2019, p. 28-35.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The hydrodynamic motion of Nanodiscs
AU - Camp, Tyler
AU - McLean, Mark
AU - Kato, Mallory
AU - Cheruzel, Lionel
AU - Sligar, Stephen
PY - 2019/5
Y1 - 2019/5
N2 - We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.
AB - We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.
KW - Nanodiscs
KW - Rotational motion
UR - http://www.scopus.com/inward/record.url?scp=85062224380&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062224380&partnerID=8YFLogxK
U2 - 10.1016/j.chemphyslip.2019.02.008
DO - 10.1016/j.chemphyslip.2019.02.008
M3 - Article
C2 - 30802435
AN - SCOPUS:85062224380
VL - 220
SP - 28
EP - 35
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
ER -