@article{7375ccffb5c04479a5b16bf52816c91b,
title = "The Hippo pathway regulates axis formation and morphogenesis in Hydra",
abstract = "How did cells of early metazoan organisms first organize themselves to form a body axis? The canonical Wnt pathway has been shown to be sufficient for induction of axis in Cnidaria, a sister group to Bilateria, and is important in bilaterian axis formation. Here, we provide experimental evidence that in cnidarian Hydra the Hippo pathway regulates the formation of a new axis during budding upstream of the Wnt pathway. The transcriptional target of the Hippo pathway, the transcriptional coactivator YAP, inhibits the initiation of budding in Hydra and is regulated by Hydra LATS. In addition, we show functions of the Hippo pathway in regulation of actin organization and cell proliferation in Hydra. We hypothesize that the Hippo pathway served as a link between continuous cell division, cell density, and axis formation early in metazoan evolution.",
keywords = "Hippo, Hydra, Wnt, Yap, axis formation",
author = "Maria Brooun and Willi Salvenmoser and Catherine Dana and Marius Sudol and Robert Steele and Bert Hobmayer and Helen McNeill",
note = "Funding Information: ACKNOWLEDGMENTS. We thank Joe Culotti (Lunenfeld Tanenbaum Research Institute, Toronto) for invaluable support of the project and help in writing the manuscript, Leonid Brown (University of Guelph) for help with culturing Hydra, Marina Gertsenstein (Toronto Center for Phenogenomics) for help with the elec-troporation procedure, Thomas Bosch and Alexander Klimovich (University of Kiel) for providing GFP transgenic Hydra, Taylor Skokan (University of California, San Francisco) for providing the MRLC-GFP transgenic Hydra, and Celina Juliano (University of California, Davis) for providing anti-HyWi antibody. H.M. is supported by funding from the Barnes-Jewish/Christian investigator program and is a Larry J. Shapiro and Carol-Ann Uetake-Shapiro Professor. B.H. is supported by the European Commission H2020 Marie Sklodowska-Curie COFUND research grant No. 847681 Ageing, Regeneration and Drug Research. R.S. is supported by Grant 1R24GM080537-01A1 from the National Institute of General Medical Sciences. Publisher Copyright: {\textcopyright} 2022 by the authors.",
year = "2022",
month = jul,
day = "19",
doi = "10.1073/pnas.2203257119",
language = "English (US)",
volume = "119",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "29",
}