@article{ba4468ddf0264b5ebf3b584d22490873,
title = "The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes",
abstract = "The glycyl radical enzymes (GREs) p-hydroxyphenylacetate (HPA) decarboxylase (HPAD), indoleacetate decarboxylase, and phenylacetate decarboxylase catalyze the radical-mediated decarboxylation of arylacetates, which are products of bacterial aromatic amino acid fermentation. Here, we report the discovery and structural and biochemical investigation of a fourth GRE arylacetate decarboxylase (AAD) from Olsenella scatoligenes that catalyzes HPA decarboxylation. AAD also catalyzes the decarboxylation of p-aminophenylacetate, which is not a substrate of HPAD, and lacks the Fe-S cluster containing small subunit. The structure of AAD in complex with p-hydroxyphenylacetate was determined by X-ray crystallography. The differing substrate ranges and active site structures of AAD and HPAD suggest distinct catalytic mechanisms, underscoring the diversity of radical-mediated decarboxylation reactions. ",
keywords = "anaerobic, arylacetate, crystal structure, decarboxylation, glycyl radical enzyme",
author = "Qiang Lu and Yifeng Wei and Lianyun Lin and Jiayi Liu and Yongxu Duan and Yaxin Li and Weixiang Zhai and Yangping Liu and Ang, {Ee Lui} and Huimin Zhao and Zhiguang Yuchi and Yan Zhang",
note = "Funding Information: We thank Zhi Li and Drs. Yong Zhang and Jun Xu at the instrument analytical center of the School of Pharmaceutical Science and Technology at Tianjin University for providing technical support in LC- and GC-MS analyses and in-house X-ray diffraction. We thank the staff for assistance using the X-ray facility on the beamline BL18U1 at Shanghai Synchrotron Radiation Facility. We are grateful to Xinying Wu and Prof. Suwen Zhao for the helpful discussions. This work was supported by the National Key R&D Program of China (Grant 2019YFA0905700), the National Natural Science Foundation of China (Grant 31870049) (Y.Z.), the National Natural Science Foundation of China (Grants 32022073, 31972287), the Natural Science Foundation of Tianjin (Grant 19JCYBJC24500) (Z.Y.), and the Agency for Science, Research and Technology of Singapore Visiting Investigator Program Grant 1535j00137 (H.Z.). Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",
year = "2021",
month = may,
day = "7",
doi = "10.1021/acscatal.1c01253",
language = "English (US)",
volume = "11",
pages = "5789--5794",
journal = "ACS Catalysis",
issn = "2155-5435",
publisher = "American Chemical Society",
number = "9",
}