TY - JOUR
T1 - The gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae
AU - Cronan, John E.
AU - Wallace, John C.
N1 - Funding Information:
This work was supported by a Fogarty Senior International Fellowship (TWO18161 and National Institutes of Health Grant AI15650 to J. E. C. and Australian Research Council grant A09131713 to J. C. W. We thank Dr. D. Val for performing the mapping hybridizations and Dr. P. Goodlove for DNA sequencing.
PY - 1995/8/1
Y1 - 1995/8/1
N2 - We report the isolation, genomic mapping, and DNA sequence of the BPL1 gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae. The gene was isolated by complementation of an Escherichia coli birA (biotin-apoprotein ligase) mutant indicating that the expressed yeast protein modified the essential biotinated protein of the bacterial host. The BPL1 gene encodes a protein of 690 residues (Mr 76.4 kDa) with strong sequence similarites to the E. coli and human biotin-apoprotein ligases. BPL1 was mapped to chromosome IV, is allelic to the previously described ACC2 gene, and encodes the major (if not the only) biotin-apoprotein ligase activity of S. cerevisiae.
AB - We report the isolation, genomic mapping, and DNA sequence of the BPL1 gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae. The gene was isolated by complementation of an Escherichia coli birA (biotin-apoprotein ligase) mutant indicating that the expressed yeast protein modified the essential biotinated protein of the bacterial host. The BPL1 gene encodes a protein of 690 residues (Mr 76.4 kDa) with strong sequence similarites to the E. coli and human biotin-apoprotein ligases. BPL1 was mapped to chromosome IV, is allelic to the previously described ACC2 gene, and encodes the major (if not the only) biotin-apoprotein ligase activity of S. cerevisiae.
KW - Biotin modified protein
KW - Biotin-apoprotein ligase
KW - Escherichia coli birA
KW - Saccharomyces cerevisiae
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U2 - 10.1016/0378-1097(95)00210-V
DO - 10.1016/0378-1097(95)00210-V
M3 - Article
C2 - 7649444
AN - SCOPUS:0028816609
SN - 0378-1097
VL - 130
SP - 221
EP - 229
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 2-3
ER -