The fungicide captan reduces nuclear DNA content in maize seedlings

A. Lane Rayburn, Wayne L. Pedersen, Lisa M. McMurphy

Research output: Contribution to journalArticle

Abstract

Captan, a major fungicide used by the US maize industry, was studied to determine its effect on the nuclear DNA content of maize (Zea mays L.) seedlings. Captan was applied to kernels at the recommended agronomic rates. The nuclear DNA content of the seedlings was determined by flow cytometry. In two maize hybrids examined, a reduction in nuclear DNA content was observed. Two different fluorochromes, with different staining mechanisms, were used to determine nuclear DNA content. Both of these fluorochromes revealed a reduction in nuclear DNA. Captan is reported to inhibit DNA synthesis in several animal systems. The reduction of maize nuclear DNA observed in this study may be a result of DNA synthesis inhibition induced by captan.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalPesticide Science
Volume37
Issue number1
DOIs
StatePublished - 1993

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Captan
captan
Seedlings
nuclear genome
Zea mays
fungicides
corn
seedlings
DNA
fluorescent dyes
Fluorescent Dyes
synthesis
flow cytometry
industry
Industry
Flow Cytometry
Staining and Labeling
seeds

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

The fungicide captan reduces nuclear DNA content in maize seedlings. / Rayburn, A. Lane; Pedersen, Wayne L.; McMurphy, Lisa M.

In: Pesticide Science, Vol. 37, No. 1, 1993, p. 79-82.

Research output: Contribution to journalArticle

Rayburn, A. Lane ; Pedersen, Wayne L. ; McMurphy, Lisa M. / The fungicide captan reduces nuclear DNA content in maize seedlings. In: Pesticide Science. 1993 ; Vol. 37, No. 1. pp. 79-82.
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