The flagellum of Pseudomonas aeruginosa is required for resistance to clearance by surfactant protein A

Shiping Zhang, Francis X. McCormack, Roger C. Levesque, George A. O'Toole, Gee Lau

Research output: Contribution to journalArticle

Abstract

Surfactant protein A (SP-A) is an important lung innate immune protein that kills microbial pathogens by opsonization and membrane permeabilization. We investigated the basis of SP-A-mediated pulmonary clearance of Pseudomonas aeruginosa using genetically-engineered SP-A mice and a library of signature-tagged P. aeruginosa mutants. A mutant with an insertion into flgE, the gene that encodes flagellar hook protein, was preferentially cleared by the SP-A+/+ mice, but survived in the SP-A-/- mice. Opsonization by SP-A did not play a role in flgE clearance. However, exposure to SP-A directly permeabilized and killed the flgE mutant, but not the wild-type parental strain. P. aeruginosa strains with mutation in other flagellar genes, as well as mucoid, nonmotile isolates from cystic fibrosis patients, were also permeabilized by SP-A. Provision of the wild-type fliC gene restored the resistance to SP-A-mediated membrane permeabilization in the fliC-deficient bacteria. In addition, non-mucoid, motile revertants of CF isolates reacquired resistance to SP-A-mediated membrane permeability. Resistance to SP-A was dependent on the presence of an intact flagellar structure, and independent of flagellar-dependent motility. We provide evidence that flagellar-deficient mutants harbor inadequate amounts of LPS required to resist membrane permeabilization by SP-A and cellular lysis by detergent targeting bacterial outer membranes. Thus, the flagellum of P. aeruginosa plays an indirect but important role resisting SP-A-mediated clearance and membrane permeabilization.

Original languageEnglish (US)
Article numbere564
JournalPloS one
Volume2
Issue number6
DOIs
StatePublished - Jun 27 2007

Fingerprint

Pulmonary Surfactant-Associated Protein A
Flagella
flagellum
Pseudomonas aeruginosa
surfactants
proteins
Membranes
Genes
mutants
mice
lungs
Lung
Hooks
Pathogens
Ports and harbors
genes
cystic fibrosis
Cystic Fibrosis
Detergents
membrane permeability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The flagellum of Pseudomonas aeruginosa is required for resistance to clearance by surfactant protein A. / Zhang, Shiping; McCormack, Francis X.; Levesque, Roger C.; O'Toole, George A.; Lau, Gee.

In: PloS one, Vol. 2, No. 6, e564, 27.06.2007.

Research output: Contribution to journalArticle

Zhang, Shiping ; McCormack, Francis X. ; Levesque, Roger C. ; O'Toole, George A. ; Lau, Gee. / The flagellum of Pseudomonas aeruginosa is required for resistance to clearance by surfactant protein A. In: PloS one. 2007 ; Vol. 2, No. 6.
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