The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Marta Yanina Pepino, Latisha Love-Gregory, Samuel Klein, Nada A. Abumrad

Research output: Contribution to journalArticle

Abstract

The precise orosensory inputs engaged for dietary lipids detection in humans are unknown. We evaluated whether a common single nucleotide polymorphism (rs1761667) in the CD36 gene that reduces CD36 expression and the addition of orlistat, a lipase inhibitor, to reduce FA release from triacylglycerols (TGs), the main component of dietary fats, would attenuate fat orosensory sensitivity in humans. Twenty-one obese subjects with different rs1761667 genotypes (6 AA, 7 AG, and 8 GG) were studied on two occasions in which oleic acid and triolein orosensory detection thresholds were measured using emulsions prepared with and without orlistat. Subjects homozygous for the G-allele had 8-fold lower oral detection thresholds for oleic acid and triolein than subjects homozygous for the A allele, which associates with lower CD36 expression ( P = 0.03). Thresholds for heterozygous subjects were intermediate. The addition of orlistat increased detection thresholds for triolein (log threshold = -0.3 ± 0.2 vs. 0.3 ± 0.1; P < 0.001) but not oleic acid (log threshold = -1.0 ± 0.2 vs. -0.8 ± 0.2; P > 0.2). In conclusion, this is the first experimental evidence for a role of CD36 in fat gustatory perception in humans. The data also support involvement of lingual lipase and are consistent with the concept that FA and not TG is the sensed stimulus.

Original languageEnglish (US)
Pages (from-to)561-566
Number of pages6
JournalJournal of Lipid Research
Volume53
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Triolein
Lipase
Tongue
Fatty Acids
Genes
Fats
Oleic Acid
Triglycerides
Alleles
Taste Perception
Dietary Fats
Emulsions
Polymorphism
Single Nucleotide Polymorphism
Nucleotides
Genotype
Lipids
orlistat
pregastric esterase

Keywords

  • Diet and dietary lipids
  • Fat oral sensitivity
  • Genetics
  • Lipids
  • Obesity
  • Taste perception
  • Triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects. / Pepino, Marta Yanina; Love-Gregory, Latisha; Klein, Samuel; Abumrad, Nada A.

In: Journal of Lipid Research, Vol. 53, No. 3, 01.03.2012, p. 561-566.

Research output: Contribution to journalArticle

Pepino, Marta Yanina ; Love-Gregory, Latisha ; Klein, Samuel ; Abumrad, Nada A. / The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects. In: Journal of Lipid Research. 2012 ; Vol. 53, No. 3. pp. 561-566.
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