The external limiting membrane in developing normal and dysplastic canine retina

Herbert E. Whiteley, Stuart Young

Research output: Contribution to journalArticlepeer-review

Abstract

Development of the external limiting membrane of the retina in English springer spaniel fetuses and neonates with incipient stages of retinal dysplasia and in normal mongrel fetuses and neonates was examined using transmission electron microscopy and the freeze-fracture technique. The external limiting membrane of the retina of normal canine fetuses was composed of zonulae adherentes in which there were focal areas of apparent apposition between adjacent ventricular cell membranes. Freeze-fracture examination revealed gap junctions within the external limiting membrane that were composed of 8-10 nm P-face particles in aggregates of variable size and shape. These junctions corresponded to areas of focal membrane apposition. Tight junction strands were also identified in deep E-face grooves which were located perpendicular to the external limiting membrane. At day 46 of gestation in incipiently dysplastic English springer spaniel fetal eyes, there was a marked decrease in the size and area occupied by gap junctions within the external limiting membrane as compared with retinas of mongrel control fetuses at the same age and of other age groups. This apparent loss of gap junctions, coincident with the incipient onset of histologically detectable dysplastic events in the sensory retina, may contribute to the morphogenesis of the defect.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalTissue and Cell
Volume18
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • Retinal dysplasia
  • canine
  • external limiting membrane
  • freeze-fracture
  • gap junctions

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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