The estrogen cytosol receptor of female ovine pituitary

Phyllis M. Wise, Anita H. Payne

Research output: Contribution to journalArticlepeer-review

Abstract

The biochemical parameters of estrone and estradiol binding to the cytosol fraction of ovine anterior pituitary were investigated. When increasing amounts of [3H]estrone or [3H]estradiol were incubated with the 105,000 g fraction from the pituitary, both hormones bound to a receptor with the same apparent KD (mean ± S.E., estrone = 1.40 ± 0.30 × 10-10 M, estradiol = 1.03 ± 0.11 x 10-10M) and the same concentration of binding sites (estrone = 3.22 ± 0.58 × 10-14 moles/mg protein, estradiol = 3.92 ± 0.19 × 10-14). No conversion of [3H]estrone to [3H]estradiol under the experimental conditions used could be demonstrated. The receptor-estrogen complex exhibited identical sedimentation coefficients (7-8 S) with either hormone. The receptor was specific only for estrogens; neither 500-fold excess of testosterone nor progesterone affected binding. Competitive inhibition using increasing amounts of non-radioactive estrone or estradiol with [3H]estrone or [3H]estradiol resulted in parallel displacement of the radioactive hormone. These results strongly suggest that both hormones bind to the same pituitary cytosol receptor.

Original languageEnglish (US)
Pages (from-to)385-395
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume3
Issue number5
DOIs
StatePublished - Nov 1975
Externally publishedYes

Keywords

  • 17β-estradiol
  • anterior pituitary
  • cytoplasmic receptor
  • estrone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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