The essential genome of the crenarchaeal model Sulfolobus islandicus

Changyi Zhang, Alex P.R. Phillips, Rebecca L. Wipfler, Gary J. Olsen, Rachel J. Whitaker

Research output: Contribution to journalArticle

Abstract

Sulfolobus islandicus is a model microorganism in the TACK superphylum of the Archaea, a key lineage in the evolutionary history of cells. Here we report a genome-wide identification of the repertoire of genes essential to S. islandicus growth in culture. We confirm previous targeted gene knockouts, uncover the non-essentiality of functions assumed to be essential to the Sulfolobus cell, including the proteinaceous S-layer, and highlight essential genes whose functions are yet to be determined. Phyletic distributions illustrate the potential transitions that may have occurred during the evolution of this archaeal microorganism, and highlight sets of genes that may have been associated with each transition. We use this comparative context as a lens to focus future research on archaea-specific uncharacterized essential genes that may provide valuable insights into the evolutionary history of cells.

Original languageEnglish (US)
Article number4908
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

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Sulfolobus
genome
Essential Genes
genes
Genes
Archaea
Genome
History
microorganisms
Gene Knockout Techniques
Microorganisms
Lenses
cells
histories
Growth
Cell culture
lenses

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The essential genome of the crenarchaeal model Sulfolobus islandicus. / Zhang, Changyi; Phillips, Alex P.R.; Wipfler, Rebecca L.; Olsen, Gary J.; Whitaker, Rachel J.

In: Nature communications, Vol. 9, No. 1, 4908, 01.12.2018.

Research output: Contribution to journalArticle

Zhang, Changyi ; Phillips, Alex P.R. ; Wipfler, Rebecca L. ; Olsen, Gary J. ; Whitaker, Rachel J. / The essential genome of the crenarchaeal model Sulfolobus islandicus. In: Nature communications. 2018 ; Vol. 9, No. 1.
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