The Enzymology of Prochlorosin Biosynthesis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lanthipeptides are ribosomally synthesized and posttranslationally modified peptides containing thioether cross-links formed through addition of a cysteine to a dehydroalanine (to form lanthionine) or to a dehydrobutyrine (to form 3-methyllanthionine). Genome sequencing of marine cyanobacteria lead to the discovery of 1.6 million open reading frames encoding lanthipeptides. In many cases, a genome encodes a single lanthipeptide synthetase, but a large number of substrates. The enzymatic modification process in Prochlorococcus MIT9313 has been reconstituted in vitro, and a variety of experimental approaches have been used to try and understand how one enzyme is capable of modifying 30 different substrates. The methods used to characterize this system will be described along with a brief genomic description of the lanthipeptide landscape found in Prochlorococcus and Synechococcus.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsBradley S. Moore
PublisherAcademic Press Inc.
Pages165-203
Number of pages39
ISBN (Print)9780128139592
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Enzymology
Volume604
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Prochlorococcus
Biosynthesis
Genes
Genome
Synechococcus
Cyanobacteria
Sulfides
Substrates
Ligases
Open Reading Frames
Cysteine
Peptides
Enzymes

Keywords

  • Combinatorial biosynthesis
  • Lanthipeptide
  • Macrocyclic peptides
  • Marine cyanobacteria
  • Prochlorococcus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bobeica, S. C., & van der Donk, W. A. (2018). The Enzymology of Prochlorosin Biosynthesis. In B. S. Moore (Ed.), Methods in Enzymology (pp. 165-203). (Methods in Enzymology; Vol. 604). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2018.01.038

The Enzymology of Prochlorosin Biosynthesis. / Bobeica, Silvia C.; van der Donk, Wilfred Adrianus.

Methods in Enzymology. ed. / Bradley S. Moore. Academic Press Inc., 2018. p. 165-203 (Methods in Enzymology; Vol. 604).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bobeica, SC & van der Donk, WA 2018, The Enzymology of Prochlorosin Biosynthesis. in BS Moore (ed.), Methods in Enzymology. Methods in Enzymology, vol. 604, Academic Press Inc., pp. 165-203. https://doi.org/10.1016/bs.mie.2018.01.038
Bobeica SC, van der Donk WA. The Enzymology of Prochlorosin Biosynthesis. In Moore BS, editor, Methods in Enzymology. Academic Press Inc. 2018. p. 165-203. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2018.01.038
Bobeica, Silvia C. ; van der Donk, Wilfred Adrianus. / The Enzymology of Prochlorosin Biosynthesis. Methods in Enzymology. editor / Bradley S. Moore. Academic Press Inc., 2018. pp. 165-203 (Methods in Enzymology).
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