The emerging landscape of single-molecule protein sequencing technologies

Javier Antonio Alfaro; Peggy Bohländer; Mingjie Dai; Mike Filius; Cecil J. Howard; Xander F. van Kooten; Shilo Ohayon; Adam Pomorski; Sonja Schmid; Aleksei Aksimentiev; Eric V. Anslyn; Georges Bedran; Chan Cao; Mauro Chinappi; Etienne Coyaud; Cees Dekker; Gunnar Dittmar; Nicholas Drachman; Rienk Eelkema; David Goodlett; Sébastien Hentz; Umesh Kalathiya; Neil L. Kelleher; Ryan T. Kelly; Zvi Kelman; Sung Hyun Kim; Bernhard Kuster; David Rodriguez-Larrea; Stuart Lindsay; Giovanni Maglia; Edward M. Marcotte; John P. Marino; Christophe Masselon; Michael Mayer; Patroklos Samaras; Kumar Sarthak; Lusia Sepiashvili; Derek Stein; Meni Wanunu; Mathias Wilhelm; Peng Yin; Amit Meller; Chirlmin Joo

doi:10.1038/s41592-021-01143-1

The emerging landscape of single-molecule protein sequencing technologies

Javier Antonio Alfaro, Peggy Bohländer, Mingjie Dai, Mike Filius, Cecil J. Howard, Xander F. van Kooten, Shilo Ohayon, Adam Pomorski, Sonja Schmid, Aleksei Aksimentiev, Eric V. Anslyn, Georges Bedran, Chan Cao, Mauro Chinappi, Etienne Coyaud, Cees Dekker, Gunnar Dittmar, Nicholas Drachman, Rienk Eelkema, David GoodlettSébastien Hentz, Umesh Kalathiya, Neil L. Kelleher, Ryan T. Kelly, Zvi Kelman, Sung Hyun Kim, Bernhard Kuster, David Rodriguez-Larrea, Stuart Lindsay, Giovanni Maglia, Edward M. Marcotte, John P. Marino, Christophe Masselon, Michael Mayer, Patroklos Samaras, Kumar Sarthak, Lusia Sepiashvili, Derek Stein, Meni Wanunu, Mathias Wilhelm, Peng Yin, Amit Meller, Chirlmin Joo

Research output: Contribution to journal › Review article › peer-review

Abstract

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

Original language	English (US)
Pages (from-to)	604-617
Number of pages	14
Journal	Nature Methods
Volume	18
Issue number	6
DOIs	https://doi.org/10.1038/s41592-021-01143-1
State	Published - Jun 2021
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/s41592-021-01143-1

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Alfaro, J. A., Bohländer, P., Dai, M., Filius, M., Howard, C. J., van Kooten, X. F., Ohayon, S., Pomorski, A., Schmid, S., Aksimentiev, A., Anslyn, E. V., Bedran, G., Cao, C., Chinappi, M., Coyaud, E., Dekker, C., Dittmar, G., Drachman, N., Eelkema, R., ... Joo, C. (2021). The emerging landscape of single-molecule protein sequencing technologies. Nature Methods, 18(6), 604-617. https://doi.org/10.1038/s41592-021-01143-1

Alfaro, JA, Bohländer, P, Dai, M, Filius, M, Howard, CJ, van Kooten, XF, Ohayon, S, Pomorski, A, Schmid, S, Aksimentiev, A, Anslyn, EV, Bedran, G, Cao, C, Chinappi, M, Coyaud, E, Dekker, C, Dittmar, G, Drachman, N, Eelkema, R, Goodlett, D, Hentz, S, Kalathiya, U, Kelleher, NL, Kelly, RT, Kelman, Z, Kim, SH, Kuster, B, Rodriguez-Larrea, D, Lindsay, S, Maglia, G, Marcotte, EM, Marino, JP, Masselon, C, Mayer, M, Samaras, P, Sarthak, K, Sepiashvili, L, Stein, D, Wanunu, M, Wilhelm, M, Yin, P, Meller, A & Joo, C 2021, 'The emerging landscape of single-molecule protein sequencing technologies', Nature Methods, vol. 18, no. 6, pp. 604-617. https://doi.org/10.1038/s41592-021-01143-1

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abstract = "Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.",

author = "Alfaro, {Javier Antonio} and Peggy Bohl{\"a}nder and Mingjie Dai and Mike Filius and Howard, {Cecil J.} and {van Kooten}, {Xander F.} and Shilo Ohayon and Adam Pomorski and Sonja Schmid and Aleksei Aksimentiev and Anslyn, {Eric V.} and Georges Bedran and Chan Cao and Mauro Chinappi and Etienne Coyaud and Cees Dekker and Gunnar Dittmar and Nicholas Drachman and Rienk Eelkema and David Goodlett and S{\'e}bastien Hentz and Umesh Kalathiya and Kelleher, {Neil L.} and Kelly, {Ryan T.} and Zvi Kelman and Kim, {Sung Hyun} and Bernhard Kuster and David Rodriguez-Larrea and Stuart Lindsay and Giovanni Maglia and Marcotte, {Edward M.} and Marino, {John P.} and Christophe Masselon and Michael Mayer and Patroklos Samaras and Kumar Sarthak and Lusia Sepiashvili and Derek Stein and Meni Wanunu and Mathias Wilhelm and Peng Yin and Amit Meller and Chirlmin Joo",

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doi = "10.1038/s41592-021-01143-1",

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AU - Alfaro, Javier Antonio

AU - Bohländer, Peggy

AU - Dai, Mingjie

AU - Filius, Mike

AU - Howard, Cecil J.

AU - van Kooten, Xander F.

AU - Ohayon, Shilo

AU - Pomorski, Adam

AU - Schmid, Sonja

AU - Aksimentiev, Aleksei

AU - Anslyn, Eric V.

AU - Bedran, Georges

AU - Cao, Chan

AU - Chinappi, Mauro

AU - Coyaud, Etienne

AU - Dekker, Cees

AU - Dittmar, Gunnar

AU - Drachman, Nicholas

AU - Eelkema, Rienk

AU - Goodlett, David

AU - Hentz, Sébastien

AU - Kalathiya, Umesh

AU - Kelleher, Neil L.

AU - Kelly, Ryan T.

AU - Kelman, Zvi

AU - Kim, Sung Hyun

AU - Kuster, Bernhard

AU - Rodriguez-Larrea, David

AU - Lindsay, Stuart

AU - Maglia, Giovanni

AU - Marcotte, Edward M.

AU - Marino, John P.

AU - Masselon, Christophe

AU - Mayer, Michael

AU - Samaras, Patroklos

AU - Sarthak, Kumar

AU - Sepiashvili, Lusia

AU - Stein, Derek

AU - Wanunu, Meni

AU - Wilhelm, Mathias

AU - Yin, Peng

AU - Meller, Amit

AU - Joo, Chirlmin

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AB - Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

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The emerging landscape of single-molecule protein sequencing technologies

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