Lipogenesis from acetate-14C (A-14C), mevalonic acid-14C (M-14C), and isopentenyl pyrophosphate-14C (IPP-14C), with or without vitamin A, was studied in perfused rat liver post-mitochondrial supernatants. The incorporation of 14C into lipid fractions after addition of 4, 40, or 400 μM retinol (OL), retinyl acetate (YL) or retinoic acid (OIC) was monitored. All forms of vitamin A depressed 14C incorporation of A-14C and M-14C into cholesterol, whereas, only OIC depressed IPP-14C accumulation into cholesterol. OIC significantly increased incorporation of activity from M-14C into the triglyceride fraction. Sodium deoxycholic acid increased A-14C but depressed IPP-14C incorporation into cholesterol. These results suggest that OL and YL act similarly, perhaps by inhibiting the conversion of M-14C to the IPP-14C. OIC may block a latter step such as the cyclization of squalene.
|Original language||English (US)|
|Number of pages||11|
|Journal||Nutrition Reports International|
|State||Published - 1977|
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science